中国当代儿科杂志Issue(10)：1124-1130,7.DOI:10.7499/j.issn.1008-8830.2015.10.021
宫内生长迟缓和高脂饮食对大鼠血脂及肝脏相关基因转录水平的影响
Effects of intrauterine growth restriction and high-fat diet on serum lipid and transcriptional levels of related hepatic genes in rats
摘要
Abstract
ObjectiveTo study the effects of intrauterine growth restriction (IUGR) and high-fat diet on the growth, lipid metabolism, and related hepatic genes in rat offspring.MethodsThe rat model of IUGR was established by food restriction during the entire pregnancy. After weaning, 32 normal rats and 24 offspring rats with IUGR were randomly allocated to standard diet group or high-fat diet group. At the age of 10 weeks, fasting plasma glucose and blood lipid were examined. Additionally, pathological sections for hepatic tissues were observed, and the transcriptional levels of related hepatic genes were measured.ResultsAt the age of 10 weeks, there was a signiifcant difference in body weight between IUGR rats and normal rats on standard diets, but no signiifcant difference in body weight was observed between the two groups on high-fat diets. Compared with the normal rats, IUGR rats showed increased energy intake and increased levels of fasting plasma glucose, total cholesterol, and triglyceride on both standard and high-fat diets. High-fat diets reduced the concentration of serum triglyceride in both normal rats and IUGR rats. IUGR and high-fat diets aggravated the fat accumulation in the liver. Two-factor analysis of variance showed that at the age of 10 weeks, the expression of genes related to lipid metabolism in the liver, PGC-1α, CPT-1, SREBF-2, HMGR, LDLR and SREBF-1, differed signiifcantly between IUGR and normal rats. Compared with standard diets, high-fat dietsincreased the expression of PPARα, SREBF-1, SREBF-2, ABCG5, and CYP7A1in both normal rats and IUGR rats. IUGR andhigh-fat diets had an interactive effect on LDLR expression.ConclusionsHyperlipidemia and fat accumulation in the liver observed in IUGR rats may be related to increased appetite and regulation disorder in genes related to fatty acid oxidation at the transcriptional level. High-fat diets may aggravate fat accumulation in the liver in rats, which may be related to increased expression of genes related to regulation of fatty acid synthesis at the transcriptional level and reduction in secretion of triglyceride.关键词
宫内生长迟缓/脂代谢/高脂饮食/大鼠Key words
Intrauterine growth restriction/Lipid metabolism/High-fat diet/Rats引用本文复制引用
陈联辉,梁黎,朱伟芬,王应旻,朱建芳..宫内生长迟缓和高脂饮食对大鼠血脂及肝脏相关基因转录水平的影响[J].中国当代儿科杂志,2015,(10):1124-1130,7.基金项目
国家自然科学基金（81170733）；国家科技支撑计划（2012BAI02B03）。 （）
