中国动物检疫Issue(5)：42-45,46,5.
病毒性出血性败血症病毒（VHSV）实时荧光环介导等温扩增检测方法的建立
Establishment of Real-time Fluorescence LAMP Assay for Viral Hemorrhagic Septicemia Virus
摘要
Abstract
A real-time loop-mediated isothermal amplification(LAMP)assay was developed for detecting viral hemorrhagic septicemia virus(VHSV). Three sets of LAMP primers were designed based on the VHSV glyG sequence,and the best one(VHSV-2) was chosen to introduce its loop primer to speed up the reaction rate.Specificity and sensitivity of the assay was evaluated using an ESE-Quant tube scanner system.The amplification temperature was 63 ℃. The results demonstrated that the amplification could be finished within 30 min.The real time LAMP assay was able to specifically detect the VHSV without reaction with other fish virus RNA. The detection limit was 4.5 pg per reaction. The results in this study suggested that the real-time LAMP method had great potential as a field molecular tool for diagnosis of VHSV. It could provide an alternative diagnostic method for field detection of VHSV.关键词
病毒性出血性败血症病毒/恒温实时荧光/环介导等温扩增Key words
Viral hemorrhagic septicemia virus/Isothermal realtimefluorescence assay分类
农业科技引用本文复制引用
..病毒性出血性败血症病毒（VHSV）实时荧光环介导等温扩增检测方法的建立[J].中国动物检疫,2013,(5):42-45,46,5.基金项目
2011年东莞市科技计划资助项目 （）
