中国肺癌杂志Issue(8)：391-399,9.DOI:10.3779/j.issn.1009-3419.2013.08.01
人肺腺癌脑转移动物模型建立及显像的研究
An Experimental Study on the Chinese Lung Adenocarcinoma Cell Clone CPA-Yang1-BR with Brain Metastasis Potency in Nude Mice and In Vivo Imaging Research
摘要
Abstract
Background and objective Lung cancer is the leading cause of cancer-related death in men and wom-en. It is also the most common cause of brain metastases. A brain metastasis model is diffcult to be established because of the presence of the blood-brain barrier (BBB) and the lack of optimal methods for detecting brain metastasis in nude mice. hTus, the establishment of a Chinese lung adenocarcinoma cell line and its animal model with brain metastasis potency and in vivo research is of great signiifcance. Methods CPA-Yang1 cells were obtained from a patient with human lung adeno-carcinoma by lentiviral vector-mediated transfection of green lfuorescence protein. Intracardiac inoculation of the cells was performed in nude mice, and brain metastatic lesions were detected using micro 18F FDG-PET/CT scanners, small animal in vivo imaging system for lfuorescence, radionuclide and X ray fused imaging, magnetic resonance imaging (MRI) with sense body detection, and resection. hTe samples were divided into two parts for cell culture and histological diagnosis. hTe process was repeated in vivo and in vitro for four cycles to obtain a novel cell clone, CPA-Yang1-BR. Results A novel cell clone, CPA-Yang1-BR, was obtained with a brain metastatic rate of 50%. hTe use of MRI for the detection of brain metasta-ses has obvious advantages. Conclusion An experimental Chinese lung adenocarcinoma cell clone (CPA-Yang1-BR) and its animal model with brain metastasis potency in nude mice were established. MRI with sense body or micro MRI may be used as a sensitive, accurate, and noninvasive method to detect experimental brain metastases in intact live immunodeifcient mice. hTe results of this study may serve as a technical platform for brain metastases from lung adenocarcinoma.关键词
肺肿瘤/肿瘤转移/脑/动物模型/PET/MRIKey words
Lung neoplasms/Tumor metastasis/Brain/Animal model/Positron emission tomography (PET)/Magnetic resonance imaging (MRI)引用本文复制引用
雷贝,曹杰,沈杰,赵兰香,梁胜,孟庆刚,谢文晖,杨顺芳..人肺腺癌脑转移动物模型建立及显像的研究[J].中国肺癌杂志,2013,(8):391-399,9.基金项目
本研究受上海市科学技术委员会创新行动计划实验动物项目（No.09140901500）资助 （）
