中国肺癌杂志Issue(11)：668-673,6.DOI:10.3779/j.issn.1009-3419.2015.11.02
Anip973细胞上清液增加脐静脉内皮细胞的生物活性
The Supernatant Obtained from Cultured Anip973 Cells Enhances the Biological Activities of HUVEC
摘要
Abstract
Background and objectiveUnlike normal tissue-derived microvascular endothelial cells, tumor mi-crovessel endothelial cells are highly reactive to growth factors and exhibit more adhesion molecules. hTus, vascular tumors are highly permeable and grow vigorously; this occurrence results in rapid growth and metastasis cancer cells. hTerefore, under-standing the characteristics of endothelial cells in the tumor microenvironment guides anti-angiogenic therapy. To this end, we explore the effect of the supernatant obtained from cultured Anip973 cells (high-metastatic human lung adenocarcinoma cells) on the biological behavior and on the cell surface markers of the human umbilical vein endothelial cell (HUVEC).Methods hTe HUVEC that was cultured in a medium (RPMI-1640 + 10% fetal bovine serum) containing various concentrations of Anip973 supernatants was categorized into experimental groups. hTe HUVEC cultured in a medium without Anip973 super-natants served as the control group. Proliferation was determined with CCK-8; blood vessel formation was investigated with three-dimensional culture techniques in vitro; and HUVEC migration was observed via transwell assay. At the same time, the expressions of CD105, CD31, and the apoptotic marker of Annexin V were detected through lfow cytometry for analyzing the relationship between the expression of cell surface markers and biological behavior.Results Following incubation with the supernatant obtained from cultured Anip973 cells, HUVEC proliferated more than the control group did, and the prolifera-tion rate was maximized when incubated in a supernatant concentration of 250 μL/mL for 24 h (P=0.002). In addition, the experimental groups exhibited varying degrees of migration and forms of vascular lumen sample structure, especially at super-natant concentrations of 125 µL/mL (P<0.001) and 250 µL/mL (P=0.002), respectively. CD105 expression was optimized at 250 μL/mL (P=0.028), and CD31 expression also increased with an increase in concentration. However, the percentage of apoptotic cells decreased. Correlation analysis results showed that cell proliferation, migration, and CD105 expression were signiifcantly and positively correlated with one another. By contrast, no signiifcant correlation was detected between CD31 expression and biological behavior.ConclusionAnip973 supernatants can promote HUVEC proliferation and migration, as well as angiogenesis. In addition, cell surface markers can change concurrently and relatively. To a certain extent, changes in CD105 expression can be attributed to shitfs in its biological behavior.关键词
Anip973/HUVEC/细胞增殖/迁移/成管/CD105/CD31/Annexin VKey words
Anip973/HUVEC/Proliferation/Migration/Tube formation/CD105/CD31/Annexin V引用本文复制引用
张翠翠,李月雅,王晶,李凯..Anip973细胞上清液增加脐静脉内皮细胞的生物活性[J].中国肺癌杂志,2015,(11):668-673,6.基金项目
本研究受天津市科委课题（No.11JCYBJC11300）、国家自然科学基金（No.81372517）、国家科技重大专项（No.2013ZX09303001）、院级课题（No.1317）和CSCO-先声抗肿瘤血管靶向治疗科研基金（No.Y-S2014-011）资助hTis study was supported by the grants from Tianjin Science & Technology Council (to Kai LI)(No.11JCYBJC11300) （to Kai LI）
National Natural Science Foundation of China (to Kai LI)(No.81372517) （to Kai LI）
National Key S&T Special Projects (to Kai LI)(No.2013ZX09303001) （to Kai LI）
Research Projects (to Cuicui ZHANG)(No.1317) and (to Jing WANG)(No.Y-S2014-011) （to Cuicui ZHANG）
