中国感染控制杂志Issue(9)：577-581,5.DOI:10.3969/j.issn.1671-9638.2015.09.001
ICU 患者血流感染耐碳青霉烯类鲍曼不动杆菌同源性分析
Homology analysis on carbapenem-resistant Acinetobacter baumannii isolated from patients with bloodstream infection in intensive care unit
摘要
Abstract
Objective To study the homology of carbapenem-resistant Acinetobacter baumannii (CRAB)isolated from patients with bloodstream infection in an intensive care unit (ICU).Methods CRAB isolated from blood-stream,lower respiratory tract,and venous catheter tips of patients with bloodstream infection in January-December 2012 were collected and performed antimicrobial susceptibility testing,homology of isolates were identified by pulsed-field gel electrophoresis (PFGE).Results 50 CRAB strains were isolated from patients’bloodstream(n =26),lower respiratory tract (n =20),and venous catheters (n =4);the resistant rates of CRAB to piperacillin, piperacillin/tazobactam,ampicillin / sulbactam,ceftriaxone,cefotaxime,cefepime,imipenem,meropenem,and ciprofloxacin were all 100%.36 CRAB isolates from patients’bloodstream (n=26),respiratory tract (n=6),and venous catheter tips(n=4)were performed PFGE,7 major PFGE genotypes(A-G)were identified,including clone A (n=5 ),B(n =2),C(n =3),D(n =4),E(n =3),F(n =8),and G(n =3 );the other 8 isolates were sporadic strains.This ICU was divided into section Ⅰand Ⅱ,clone A,B,and F all existed in both sections.CRAB isolated from different sites of 3 patients were of the same clone,2 of these patients isolated the same clone of CRAB from bloodstream,lower respiratory tract,and venous catheter tips,1 isolated the same clone of CRAB from bloodstream and lower respiratory tract.PFGE genotyping of CRAB from 6 patients’lower respiratory tract revealed that 3 strains were clone D.Venous catheter tips of 12 patients were performed culture,4 were positive for CRAB,and CRAB was of the same clone as that from patient’s respective bloodstream.Conclusion CRAB bloodstream infec-tion prevail in ICU,cross transmission among patients is the main mode of hospital epidemic.关键词
鲍曼不动杆菌/耐碳青霉烯类鲍曼不动杆菌/血流感染/静脉导管/脉冲场凝胶电泳/流行/医院感染Key words
Acinetobacter baumannii/carbapenem-resistant Acinetobacter baumannii/bloodstream infection/venous catheter/pulsed-field gel electrophoresis/epidemic/healthcare-associated infection分类
医药卫生引用本文复制引用
毛璞,李建春,叶丹,刘晓青,何为群,黎毅敏..ICU 患者血流感染耐碳青霉烯类鲍曼不动杆菌同源性分析[J].中国感染控制杂志,2015,(9):577-581,5.基金项目
广州市教育局创新团队科研基金（B94117）；广州市科技计划项目 （）
