中国康复理论与实践Issue(2)：114-118,5.DOI:10.3969/j.issn.1006-9771.2013.02.005
电针对大鼠局灶性脑缺血再灌注后脑内血管内皮生长因子受体-2 mRNA和脑源性神经营养因子蛋白表达的影响
Effect of Electroacupuncture on Expression of Vascular Endothelial Growth Factor Receptor-2 mRNA and Brain-Derived Neuro-trophic Factor Protein in Brain Tissue of Rats with Focal Cerebral Ischemia/Reperfusion
摘要
Abstract
Objective To observe the effect of electroacupuncture on the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) mRNA and brain-derived neurotrophic factor (BDNF) protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Methods The focal cerebral ischemia/reperfusion model was established with filament occlusion. 90 rats were randomly divided into control group (n=6), model group (n=42) and electroacupuncture group (n=42). They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after isch-emia/reperfusion, 6 rats in each points. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of VEG-FR-2 mRNA. Immunohistology method was used to detect the expression of BDNF protein. Results The expression of VEGFR-2 mRNA was higher in the electroacupuncture group than in the model group 12 h, 24 h, 3 d after reperfusion (P<0.05). The expression of BDNF pro-tein in the hippocampus increased 2 h (P<0.05), reached peak 24 h (P<0.01) and still higher 3 d (P<0.05) in the electroacupuncture group than in the model group after reperfusion. Conclusion Electroacupuncture can upregulate the expression of VEGFR-2 mRNA and BDNF protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Neuro-angiogenic factor is possibly the molecular basis of coordinat-ed interaction of the nervous and vascular system.关键词
缺血/再灌注/神经血管因子/血管内皮生长因子受体-2/脑源性神经营养因子/电针/大鼠Key words
ischemia/reperfusion/neuro-angiogenic factors/vascular endothelial growth factor receptor-2/brain-derived neurotrophic factor/electroacupuncture/rats分类
医药卫生引用本文复制引用
马璟曦,罗勇,蔡敏..电针对大鼠局灶性脑缺血再灌注后脑内血管内皮生长因子受体-2 mRNA和脑源性神经营养因子蛋白表达的影响[J].中国康复理论与实践,2013,(2):114-118,5.基金项目
1.教育部“高等学校博士学科点专项科研基金”(No.20095503110001)；2.重庆市卫生局中医药科研项目(No.2005-B-24)。 （No.20095503110001）
