中国康复理论与实践Issue(4)：318-323,6.DOI:10.3969/j.issn.1006-9771.2013.04.002
生物活性材料支架诱导成年大鼠神经再生修复脑损伤的研究①
Repair of Injured Adult Rat Brain by Inducing Neural Regeneration with Bioactive Material Scaffolds
摘要
Abstract
Objective To repair the brain of adult rats with traumatic brain injury by inducing neural regeneration with biological scaf-folds. Methods 52 adult male Wistar rats were divided into control group, lesion group, blank chitosan carriers group and bioactive material scaffolds group. The neural regeneration and the origin of the newborn neurons in the injury area were detected through histochemistry, im-mumochemistry and neural tracing on the 3rd day, 7th day, 2nd week, 4th week, 2nd month and 3rd month after operation. Results After ap-plication of bioactive material scaffolds, the BrdU+/Nestin+ and BrdU+/Dcx+ cells in gyrus dentatus (DG) of hippocampus and subventricular zone (SVZ) were significantly more than those of the other groups. The BrdU+/Nestin+, BrdU+/Dcx+ and BrdU+/MAP2+ neural cells in injury area of the bioactive material scaffolds group were significantly more than those of the other groups. After application of DiI to label the SVZ cells, DiI+/NeuN+ neurons were observed in the injury areas. Conclusion Bioactive material scaffolds can activate the neural stem cells in SVZ and DG. The neural stem cells in SVZ can migrate to the injury area and then differentiate into mature neurons.关键词
脑损伤/生物活性材料支架/神经干细胞/神经再生/迁移/分化/大鼠Key words
brain injury/bioactive material scaffolds/neural stem cells/neural regeneration/migrate/differentiation/rats分类
医药卫生引用本文复制引用
陈兰,孙敏,张皑峰,杨朝阳,李晓光..生物活性材料支架诱导成年大鼠神经再生修复脑损伤的研究①[J].中国康复理论与实践,2013,(4):318-323,6.基金项目
1.北京市科委重点项目(No.D09080104660000)；2.国家自然基金重点项目(No.31130022)；3.国家科技支撑计划(No.2012BAI17B04)；4.国家“863”计划(No.2012AA020506)。 （No.D09080104660000）
