中国康复理论与实践Issue(5)：426-431,6.DOI:10.3969/j.issn.1006-9771.2013.05.08
双相陶瓷生物骨-骨形态发生蛋白-碱性成纤维细胞生长因子复合物修复股骨头坏死模型的实验研究①
Biphasic Ceramic Biologic Bone, Bone Morphogenetic Protein, and Basic Fibroblast Growth Factor Complex Transplanted for Fem-oral Head Necrosis
摘要
Abstract
Objective To observe the efficacy of a kind of complex composed of biphasic ceramic biologic bone (BCBB), bone morpho-genetic protein (BMP) and basic fibroblast growth factor (bFGF) on the repair of necrotic areas of the femoral head. Methods The femoral head necrosis model of 64 femoral heads in 32 rabbits induced with microwave heating were randomly divided into four groups, which im-planted with nothing (group A), BCBB/BMP (group B), BCBB/BMP/bFGF (group C) and with cancellous bone autograft (group D). The specimens were harvested separately at the end of 2, 4, 8 and 12 weeks after operation. 4 femoral heads were taken off at each interval in ev-ery group. A series of examinations were carried out including of naked eyes and gross anatomic observation, X-ray, histology, and blood vessel immunohistochemical staining. Results In group A, 1 femoral head collapsed by the end of 12 weeks, and there was only a little oste-oid tissue formed. At the same time, a lot of new bone formed in group B and group C, and the boundary between the bone grafting area and the post bone still existed, but the boundary was unclear in group D, with the density consistent to the post bone. Under X-ray, the defect could be found and one femoral head collapsed in group A by the end of 12 weeks. The density of bone grafting area was high and the boundary to the post bone was unclear in group B and in group C. The density of bone grafting area was the same as the post bone and the boundary between them was unclear in group D. There was only a little osteoid tissue formed in group A by the end of 4 weeks. At the same time, there was a little new bone formed in group B, and BCBB was partly degraded. There was a lot of new bone formed in group C and group D, and BCBB was partly degraded in group C, but cancellous bone autograft was almost absorbed in group D. The new bone area by the end of 4, 8 and 12 weeks from more to less were:group C and group D (P>0.05), group B, and group A (P<0.05). At the end of 2, 4 and 8 weeks, the blood vessel area of group C was more than that of group A, group B, and group D (P<0.05). Conclusion The BCBB/BMP/bF-GF complex can induced osteoinduction and revascularization, to repair rabbit femoral head necrosis as effective as cancellous bone auto-graft.关键词
股骨头坏死/双相陶瓷生物骨/骨形态发生蛋白/碱性成纤维细胞生长因子/骨修复Key words
femoral head necrosis/biphasic ceramic biologic bone/bone morphogenetic protein/basic fibroblast growth factor/repair分类
医药卫生引用本文复制引用
王蕾,彭吾训,张爱华,邓进,龚跃昆,李世和,胡蕴玉..双相陶瓷生物骨-骨形态发生蛋白-碱性成纤维细胞生长因子复合物修复股骨头坏死模型的实验研究①[J].中国康复理论与实践,2013,(5):426-431,6.基金项目
1.贵州省科技厅贵阳医学院社发联合基金项目(No.黔科合[2010]3165)；2.贵州省卫生厅科技基金项目(No.gzwkj2010-1-039)。 （No.黔科合[2010]3165）
