中国康复理论与实践Issue(10)：1138-1144,7.DOI:10.3969/j.issn.1006-9771.2015.10.005
原花青素对脑缺血再灌注损伤后肠道功能的保护作用
Protective Effects of Proanthocyanidins on Intestinal Function after Cerebral Ischemia-reperfusion Injury
摘要
Abstract
Objective To explore the protective effects of proanthocyanidins pretreatment on intestinal function after cerebral isch-emia-reperfusion injury. Methods 24 Sprague-Dawley rats were randomly divided into sham group (group A, n=8), ischemia-reperfusion group (group B, n=8) and proanthocyanidins pretreatment group (group C, n=8). The model of cerebral ischemia-reperfusion injury in rats was established according to Longa's method. Group C was intraperitoneally injected with proanthocyanidins 10 mg/(kg⋅d), group A and group B were injected with normal saline for 5 consecutive days. 1 and 3 days after cerebral ischemia-reperfusion, ileum myoelectric slow wave and smooth muscle contractility, the activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) were measured, the content of the serum TNF-αwas tested with ELISA kit, ileum tissues were tested with hematoxylin eosin (HE) staining and used for mea-suring the moisture content. Results Compared with group B 1 and 3 days after cerebral ischemia-reperfusion, the intestinal mucosa injury relieved, the intestinal mucosa score decreased (P<0.05) and the number of infiltrated inflammatory cell decreased in group C;the frequency of slow wave and contraction trended to increase (P>0.05), and the amplitude increased (P<0.05) in group C;the serum SOD activity in-creased (P<0.05), and the content of MDA and TNF-αdecreased (P<0.01) in group C;the intestinal moisture content reduced (P<0.01) in group C. Conclusion Proanthocyanidins pretreatment can protect intestinal function from injury after cerebral ischemia-reperfusion.关键词
脑缺血再灌注/原花青素/肠道功能/大鼠Key words
cerebral ischemia-reperfusion/proanthocyanidins/intestinal function/rats分类
医药卫生引用本文复制引用
范加维,黄倩,杨森,杨拯,黄宏达,陈勇,宋强,罗兰,梁杰,张婷..原花青素对脑缺血再灌注损伤后肠道功能的保护作用[J].中国康复理论与实践,2015,(10):1138-1144,7.基金项目
1.国家大学生创新创业训练计划项目(No.201513705013)；2.成都医学院大学生开放实验项目(No. Kf03)。 （No.201513705013）
