中国康复理论与实践Issue(10)：1145-1150,6.DOI:10.3969/j.issn.1006-9771.2015.10.006
不同胎龄胎鼠脊髓源性神经干细胞特性比较
Differentiation of Spinal Neural Stem Cells from Various Gestational Aged Fetal Rats
摘要
Abstract
Objective To study the differentiation and proliferation ability of the spinal neural stem cells (NSCs) at different gestational ages in fetal rats. Methods Sprague-Dawley fetal rats were divided into group A (12 days of pregnancy), group B (14 days of pregnancy) and group C (16 days of pregnancy). NSCs were separated with enzyme-assisted microdissection. The diameter and numbers of NSCs balls were measured at different time. The cell growth curve was drawn with CCK8 colorimeter. NSCs were identified with BrdU/Nestin immuno-histochemical staining. They were induced with 10%fetal bovine serum for 10 days, and the expression ofβ-tubulinⅢand glial fibrillary acidic protein was detected with immunocytochemistry. Results There were cells expressed BrdU, Nestin,β-tubulinⅢand GFAP in all the group. The most cells (22.74±0.79%) expressedβ-tubulinⅢin the group B, but no significant difference between group B and group C. The cell vitality on the 5th day of third-generation neural stem cells was the most in group B. Conclusion For enzyme-assisted microdissection, it may obtain more neurons to isolate the neural stem cells from 14 days of pregnancy pregnant rats.关键词
神经干细胞/脊髓/神经元/星形胶质细胞/原浆型星形胶质细胞/大鼠Key words
neural stem cells/spinal cord/neurons/astrocyte/protoplasmic astrocyte/rats分类
医药卫生引用本文复制引用
南伟,张海鸿,冯东亮,周开升,朱彦东,汪静,田婷,江龙,龙在云,伍亚民..不同胎龄胎鼠脊髓源性神经干细胞特性比较[J].中国康复理论与实践,2015,(10):1145-1150,6.基金项目
1.国家自然科学基金项目(No.30772299)；2.甘肃省自然科学研究基金计划项目(No.1107RJZA140)。 （No.30772299）
