中国临床药理学杂志Issue(17)：1742-1744,3.DOI:10.13699/j.cnki.1001-6821.2015.17.013
载脂蛋白 E基因多态性对氟伐他汀抑制L－02细胞中3－羟基－3－甲基戊二酰辅酶 A还原酶活性的影响
Effect of Apolipoprotein E genetic polymorphism on fluvastatin inhibitory activity of 3-hydroxy-3-methyl glutaric acyl coenzyme A reductase in L-02 cell
孙旭 1罗余萍 2熊玉卿3
作者信息
- 1. 中国食品药品检定研究院 国家药物安全评价监测中心 药物非临床安全评价研究北京市重点实验室，北京 100176
- 2. 南昌大学 医学院临床药理研究所，南昌 330006
- 3. 南昌大学 医学院临床药理研究所，南昌 330006
- 折叠
摘要
Abstract
Objective To study the effect of different Apolipoprotein E isoforms on the fluvastatin inhibitory activity of 3-hydroxy-3-methyl glutaric acyl coenzyme A reductase in L -02 cells existed.Methods Enzymatic activity was evaluated by determining NADPH using HPLC method after cell sample preparation.Explored the incubation method of protein and hydroxy methylglutaryl coenzyme A reductase inhibitor ( HMG -CoA ) , as well as the inhibition effect of fluvastatin in HMG-CoA reductase activities.Results The precision , accuracy and reproducibility of the HPLC method meet the requirements.NADPH in the concentration range of 2.5-1000.0 μmol · L-1 showed good linear relationship.The fluvastatin in the concentration range of 0-5 μg· mL-1 to suppress HMG -CoA reductase activity was concentration dependent and the maximum inhibition rate was 49.73%. When Apolipoprotein E 2/3/4 exsited , the inhibition rate of fluvastatin on HMG -CoA reductase activity were 38.02%, 25.12%, 21.92%, respectively.ApoE2 showed a siganificant difference with ApoE 3.Conclusion The inhibition rate of fluvastatin in HMG -CoA reductase activities in L -02 with different Apolipoprotein E isoforms were significantly different.关键词
载脂蛋白E/基因多态性/氟伐他汀/L-02 细胞/3-羟基-3-甲基戊二酰辅酶AKey words
apolipoprotein E/genetic polymorphism/fluvastatin/L -02 cell/hydroxy methylglutaryl coenzyme A reductase inhibitor分类
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孙旭,罗余萍,熊玉卿..载脂蛋白 E基因多态性对氟伐他汀抑制L－02细胞中3－羟基－3－甲基戊二酰辅酶 A还原酶活性的影响[J].中国临床药理学杂志,2015,(17):1742-1744,3.
