中国临床药理学杂志Issue(17)：1757-1759,1785,4.DOI:10.13699/j.cnki.1001-6821.2015.17.017
雷公藤内酯醇对多发性骨髓瘤KM3细胞增殖与凋亡和H3K4me3蛋白表达的影响
Effect of triptolide on the proliferation, apoptosis and and H3K4me3 protein expression in multiple myeloma KM3 cell
摘要
Abstract
Objective To investigate the effect of triptolide on the proliferation, apoptosis and and H3K4me3 protein expression in multiple myeloma KM3 cell.Methods The MM KM3 cell line was cultured with triptolide.Cell proliferation was detected by MTT method .Apoptosis was evaluated by Annexin -V -FITC/PI -labeled flow cytometry and confocal microscopy.The expression of H3K4me3 in KM3cells was assayed by Western blotting and confocal microscopy.Results Triptolide had obvious inhibition on proliferation of KM 3 cells and showed a dose and time dependence.Triptolide had obvious inhibition on apoptosis of KM 3 cells,and with the increase of triptolide concentration , the apoptosis ratio increased gradually. The the total apoptosis rates were (48.97 ±1.78 )%, ( 53.72 ±2.21 )% and ( 60.75 ±2.43 )%.Confocal laser scanning microscopy showed H 3K4me3 accumulated in the nucleus, after 48 h intervention of 80 nmol· L-1 of triptolide, the mean value of fluorescence intensity of H3K4me3 in KM3 cells (21.96+0.34 ) was significantly lower than that in the control group ( 39.86 ±0.47 ) ( P <0.05 ) .Conclusion Triptolide inhibited cell proliferation, induced cell apoptosis, reduced the expression of H3K4me3 protein.关键词
多发性骨髓瘤/雷公藤内酯醇/KM3/组蛋白3 第4位赖氨酸的三甲基化/增殖/凋亡Key words
multiple myeloma/triptolide/KM3/H3K4me3/proliferation/apoptosis分类
医药卫生引用本文复制引用
邵燕萍,罗文达,郭群依,蔡真..雷公藤内酯醇对多发性骨髓瘤KM3细胞增殖与凋亡和H3K4me3蛋白表达的影响[J].中国临床药理学杂志,2015,(17):1757-1759,1785,4.基金项目
浙江省医药卫生科技计划基金资助项目 （）
