中国临床药理学杂志Issue(17)：1777-1780,4.DOI:10.13699/j.cnki.1001-6821.2015.17.023
siRNA抑制真核翻译延长因子1 A1表达对结肠癌细胞凋亡和药物敏感性的影响
Effect of eEF1A1 inhibition by siRNA on apoptosis and the chemosensitivity of colon carcinoma cells HCT-116
摘要
Abstract
Objective To investigate the effect of eukaryotic translation elongation factor 1A1 (eEF1A1) inhibition by siRNA on the apoptotic susceptibility , the chemosensitivity of colon carcinoma cells.Methods Four siRNA specifically targeting eEF1A1 were designed and synthesized in vitro and were transfected into colon carcinoma HCT -116 cells.Reverse transcription -polymerase chain reaction ( RT -PCR ) and Western blot assay were used to validate gene -silencing efficiency of eEF1A1.The cell apoptosis was assessed by flow cytometry ( FCM ).Cell growth state and 50% inhibition concentration ( IC50 ) of 5-fluorouracil ( 5 -FU ) and cisplatin was determined by MTT assay.Results siRNA specific to eEF1A1 can efficiently decrease the expression of eEF1 A1 in colon carcinoma HCT -116 cells.When eEF1A1 was inhibited , the reproductive activity of colon carcinoma HCT-116 cells was significantly lower than the control groups ( P<0.05).The study also showed that IC 50 of 5-FU and cisplatin on colon carcinoma HCT -116 cells treated by siRNA was decreased ( P<0.05).Conclusion The siRNA targeting eEF1A1 gene can effectively inhibit the expression of eEF1A1 gene, resulting in enhancing the apoptotic susceptibility , the chemosensitivity of 5-FU and cisplatin on colon carcinoma HCT -116 cells.关键词
siRNA/真核翻译延长因子1A1/结肠癌细胞HCT-116/凋亡/药物敏感性Key words
siRNA/eukaryotic translation elongation factor 1 A1/colon carcinoma cells HCT -116/apoptosis/chemosensitivity分类
医药卫生引用本文复制引用
苏启表,韦桂宁,刘洪俊,李辰生,廖丽贞,赵杰,李国标,李卫东..siRNA抑制真核翻译延长因子1 A1表达对结肠癌细胞凋亡和药物敏感性的影响[J].中国临床药理学杂志,2015,(17):1777-1780,4.基金项目
国家自然科学基金资助项目 （）
