中国临床药理学杂志2015，Vol.31Issue(19)：1949-1951,3.DOI:10.13699/j.cnki.1001-6821.2015.19.019
金松双黄酮联用紫杉醇对A549细胞凋亡的影响
Effects of Sciadopitysin combined with paclitaxel on the apoptosis of A549 cells
摘要
Abstract
Objective To discuss the influence of Sciadopitysin com-bined with paclitaxel on the expression of caspase -3 protein and Bcl-2, Bax genes in A549 cells.Methods The experiment was divi-ded into three groups:control , paclitaxel and sciadopitysin +paclitaxel groups.After treatment 72 h, the morphologic change was observed by phase contrast microscope .The mRNA expression of Bcl -2 and Bax was detected by reverse transcription -PCR.The expression of caspase -3 protein was analyzed by Western -blot.Results Compared with other groups , more A 549 cells treated by Sciadopitysin + paclitaxelare appeared apoptosis characteristics nuclear condensation and fragmenta-tion.Compared with paclitaxel group , in Sciadopitysin +paclitaxelare group, the mRNA level of Bcl-2 was down-regulated, while the pro-tein level of caspase -3 and mRNA level of Bax were up -regulated. Conclusion Sciadopitysin+paclitaxelare strengthened the action of pa-clitaxel to apoptosis of A549 cells, which might be correlated with the regulation of expressions of Bcl -2 , Bax and caspase -3.关键词
紫杉醇/金松双黄酮/凋亡/A549细胞Key words
paclitaxel/Sciadopitysin/apoptosis/A549 cell分类
医药卫生引用本文复制引用
赵学梅,张宏莲,孔寰宇,林宇,赵丹,崔红霞,郭丽娜..金松双黄酮联用紫杉醇对A549细胞凋亡的影响[J].中国临床药理学杂志,2015,31(19):1949-1951,3.基金项目
黑龙江省教育厅科学技术研究基金资助项目(12531834) （12531834）
