中国临床药理学杂志Issue(20)：2052-2054,3.DOI:10.13699/j.cnki.1001-6821.2015.20.022
HPLC－UV法测定商陆中商陆皂苷甲的含量
Determination of esculentoside A in Phytolaccae Radix by HPLC-UV
高立新1
作者信息
- 1. 濮阳市油田总医院 药剂科，河南 濮阳457001
- 折叠
摘要
Abstract
Objective To establish a quantitative HPLC -UV method for the determination of esculentoside A in Phytolaccae Radix.Methods The samples were analyzed using an Alltima C18 (4.6 mm ×250 mm, 5μm) column eluted with the mobile phase consisted of acetonitrile-wa-ter with 0.1 % phosphoric acid ( 40∶60 ) . The flow rate was at 0.8 mL・ min-1 and the detection wavelength was at 203 nm.Results The calibration curve for esculentoside A was linear over the range from 0.15 to 3.86μg and the limit of quantification was 0.039μg.The with-in -day and inter -day precision were expressed with 1.12% and 1.89%of RSDs.The average recovery was 98.61%.10 crude materials collected from different habitats were determined and the contents of esculentoside A in Phytolaccae Radix were fluctuated from 0.05 % to 0.42%.Conclusion The method is simple and repeatable.It could be used for the quality control of Phytolaccae Radix.关键词
商陆/商陆皂苷甲/HPLC-UVKey words
Phytolaccae Radix/esculentoside A/HPLC-UV分类
医药卫生引用本文复制引用
高立新..HPLC－UV法测定商陆中商陆皂苷甲的含量[J].中国临床药理学杂志,2015,(20):2052-2054,3.
