中国临床药理学杂志Issue(21)：2131-2133,3.DOI:10.13699/j.cnki.1001-6821.2015.21.014
HPLC指纹图谱分析血清中四逆散冻干粉的相似度
Similarity analysis of Sinisan lyophilized power in serum by HPLC fingerprint
摘要
Abstract
Objective To establish a sensitive and specific high per-formance liquid chromatography ( HPLC ) method for similarity analysis of Sinisan lyophilized power in rats serum.Methods HPLC method was applied.The chromatographic conditions were as following: Column:Kromasil C18 column ( 4.6 mm ×250 mm, 5 μm );flow -rate:1.0 mL・ min -1;column temperature:30 ℃.The mobile phase:acetonitrile ( A) and H2 O ( acidified to pH=3 with phosphoric acid) ( B) with a gra-dient elution.The concentration of solvent A was from 10% to 90% in 150 min.Ten batches Sinisan lyophilized powder samples in serum were analyzed by HPLC fingerprint.Results The HPLC chromatographic fingerprin-ting of Sinisan showed 8 characteristic peaks compared with that of blank serum, they were absorbed into blood.The result of sim-ilarity analysis was over 0.99. Conclusion The chromatographic fingerprinting of Sinisan lyophizied power had high specificity and characteristic.关键词
四逆散冻干粉/血清/高效液相色谱/指纹图谱Key words
Sinisan lyophilized power/serum/HPLC/fingerprinting分类
医药卫生引用本文复制引用
李越峰,曹瑞,徐富菊,张泽国,牛江涛,严兴科..HPLC指纹图谱分析血清中四逆散冻干粉的相似度[J].中国临床药理学杂志,2015,(21):2131-2133,3.基金项目
国家自然科学基金资助项目（81460611）；教育部科学技术研究重点基金资助项目（212186）；甘肃省自然科学基金资助项目（20101010 RJZA212；2014145 RJZA076）；甘肃省财政厅高校基本科研业务费专项基金资金项目（2013－2）；甘肃省中医药管理局科研课题基金资助项目（GZK－2015－57）；兰州市科技局基金资金项目基金资助项目 （）
