中国免疫学杂志Issue(10)：1315-1319,5.DOI:10.3969/j.issn.1000-484X.2015.10.004
丁酸对小鼠骨髓源树突状细胞的免疫调节作用
Immunomodulatory effects of butyrate on bone marrow derived dendritic cells
强叶涛 1邵启祥 1夏圣 1吴水芸 2韩慕天 1程璐 1金慧敏 2严成 2李翀 3刘霞 1张苗苗1
作者信息
- 1. 江苏大学医学院免疫学与免疫检验学系，镇江 212013
- 2. 江苏大学医学院检验医学研究所，镇江 212013
- 3. 江苏大学附属昆山医院，昆山 215300
- 折叠
摘要
Abstract
Objective:To investigate the effect of butyrate produced by bacterial metabolism on immune features of murine bone marrow-derived dendritic cells(BMDCs) and its potential mechanisms.Methods: BMDCs were prepared from bone marrow cells of C57BL/6 mice by being cultured with GM-CSF and IL-4.The expression of CD80,CD86,B7-DC and MHCⅡ on BMDCs and its pri-ming ability on the proliferation of OVA257-264 antigen specific CD8+T cell were analyzed by using Flow cytometry.The mRNA levels of IL-6 and IL-12 in BMDCs were detected by real-time fluorescence quantitative PCR(q-PCR).Simultaneously,Griess reaction and Western blot was used for analyzing the levels of NO2-in BMDCs culture supernatant and the ERK phosphortylation in BMDCs respectivly.Results:Butyrate could decrease the levels of CD80,CD86,MHCⅡand B7-DC,and downregulate the capability of BMDCs in priming the proliferation of CD8+T cells.Furthermore,the secretions of IL-6,IL-12,NO2-and the phosphorylation of ERK were sup-pressed.Conclusion:Butyrate down-regulats the immune functions of BMDCs via inhibition of ERK phosphorylation in TLR 4 signaling pathway.关键词
丁酸盐/树突状细胞/共刺激分子/细胞因子/CD8+T细胞/TLR4Key words
Butyrate/Dendritic cell/Costimulatory molecule/Cytokine/CD8+T/TLR4分类
医药卫生引用本文复制引用
强叶涛,邵启祥,夏圣,吴水芸,韩慕天,程璐,金慧敏,严成,李翀,刘霞,张苗苗..丁酸对小鼠骨髓源树突状细胞的免疫调节作用[J].中国免疫学杂志,2015,(10):1315-1319,5.
