中国免疫学杂志Issue(10)：1357-1360,1374,5.DOI:10.3969/j.issn.1000-484X.2015.10.013
N－cadherin通过β－catenin调控鸡胚脊髓连合纤维向对侧的投射
N-cadherin regulates projection of spinal commissural axons viaβ-catenin during chicken embryonic development
摘要
Abstract
Objective:To explore the role of N-cadherin and β-catenin in the formation of spinal commissural axon projection during chicken embryonic development.Methods:Fertilized eggs were cultured for three days(stage22),N-cadherin orβ-catenin inter-ference plasmid was injected into the neural tube and in vivo electroporation was performed.Three days after the electroporation, embryos were collected,fixed with 4%PFA,embeded with OCT,and cut into frozen sections.Four groups ( knockdown of N-cadherin orβ-catenin or both of them,and control) were included in this study.Immunohistochemistry method was used to analyze the protein expression result of N-cadherin or β-catenin.The changes of spinal commissural projections were observed with GFP fluorescence.Results:During chicken embryonic development,knockdown of N-cadherin inhibited the expression of β-catenin in the spinal cord.The commissural nerve fibers projecting to the contralateral side of the spinal cord was impaired after knockdown of N-cadherin or β-catenin;this phenotype was similar after knocking down both of them.Conclusion: N-cadherin is implicated in the formation of spinal commissural projection in the developing spinal cord,possibly viaβ-catenin.关键词
鸡胚/神经钙黏蛋白/β-连环蛋白/神经纤维Key words
Chicken embryonic/Neural cadherin/β-catenin/Nerve fibers分类
生物科学引用本文复制引用
杨慈清,李小英,王聪睿,张必超,李晗,林俊堂..N－cadherin通过β－catenin调控鸡胚脊髓连合纤维向对侧的投射[J].中国免疫学杂志,2015,(10):1357-1360,1374,5.基金项目
本文受2013年河南省教育厅科学技术研究重点项目（13A320866）、新乡医学院科研培育基金（2013QN125）、新乡医学院科研培育基金（2013QN115）和新乡医学院博士启动基金（505090）资助。 （）
