中国免疫学杂志Issue(11)：1490-1493,4.DOI:10.3969/j.issn.1000-484X.2015.11.010
沉默GRP78／BIP表达对人膀胱癌T24细胞侵袭及迁移的影响及机制
Effect of GRP78 silencing on invasion and migration abilities of human Bladder cancer T24 cells
摘要
Abstract
Objective:To investigate effect of silencing GRP78 in the invasion and metastasis of Bladder cancer T24 cells and the possible mechanism.Methods: The siRNA targeting GRP78 gene was chemically synthesized, and transfect into human bladder cancer T24 cells by Liopfecta mineRNAIMAX.The mRNA and protein expression levels of GRP78,MMP2,MMP9 and Timp-2 were detected by Real-time PCR and Western blot.Transwell assay and Scratch Wound Assay were conducted to deter mine the potency of migration and invasion of T24 cells.Results:The expression of AEG1 mRNA and protein were significantly down-regulated in T24 cells transfected with siRNA targeting GRP78 as compared with the control group( P<0.01);so was expression levels of MMP2 and MMP9 protein( P<0.05 );but timp-2 expression was up-regulated.Cell migration and invasion capacity of T24 cells tranfected with GRP78siRNA group had lower than the cells in the transfection with siRNA-control group(P<0.05).Conclusion:GRP78 functions as a positive regulator in the invasion and metastasis of bladder cancer cells,and may be involved in the expression of MMP2,MMP9 and Tipm-2.关键词
GRP78/膀胱癌/侵袭与迁移Key words
GRP78/Bladder cancer/Invasion and migration分类
医药卫生引用本文复制引用
伍家燕,樊建军,曾帆,李海玉,李韵,张寒韬,白鑫,刘革力,宋方洲..沉默GRP78／BIP表达对人膀胱癌T24细胞侵袭及迁移的影响及机制[J].中国免疫学杂志,2015,(11):1490-1493,4.基金项目
本文为高等学校博士学科点专项科研基金（No.20125503110012）。 （）
