中国免疫学杂志Issue(11)：1501-1504,4.DOI:10.3969/j.issn.1000-484X.2015.11.013
豫北地区嗜水气单胞菌灭活疫苗的制备及免疫效果的研究
Preparation of a deactivation vaccine against Aeromonas hydrophila and research about its immue effect in the north of Henan
摘要
Abstract
Objective:Selected virulence factors more than and high virulent Aeromonas hydrophia strain made into inactivated vaccine,to study the immunization effect of inactivated vaccine.Methods:Crucian were vaccinated with formalin-killed vaccine via in-traperitoneal injection.Controls were injected with the same volumes of saline.Then the antibody titres, histopathology and relative percent survival were analyzed from samples of both groups.Results: The antibody in the indirect agglutination reaction could be detected in vaccinated fish once a weeks after immunization and reached highest level 6 weeks after immunization.The histopathology analysis indicated that the vaccine had a good protective effect on crucian target organs.Vaccinated fish showed 100%relative percent survival and the immune period would be 6 month.Conclusion:The vaccine in this study has a significant protective effect on crucian and may be used as effective fish vaccines against bacterial septicemia.关键词
嗜水气单胞菌( Aeromonas hydrophil)/疫苗/抗体效价/病理切片/免疫保护率Key words
Aeromonas hydrophia/Vaccine/Antibody titers/Tissue slices/Immunization protection rate分类
医药卫生引用本文复制引用
关建义,毛会丽,贺文旭,杨利敏,符运栋,张敬梅,吴佳静,宁黔冀..豫北地区嗜水气单胞菌灭活疫苗的制备及免疫效果的研究[J].中国免疫学杂志,2015,(11):1501-1504,4.基金项目
本文为河南省基础与前言技术研究项目（142300410021）、河南省科技厅科技攻关（132102310165）、河南省教育厅科技攻关资助项目（2011A240001）和2013年国家大学生创新课题（201310472007）。 （）
