中国麻业科学Issue(5)：233-238,6.
悬铃叶苎麻基因组 DNA 的六种提取方法比较
Comparison of Six Methods for the Extraction of Genomic DNA from Boehmeria tricuspis (Hance)Makino
摘要
Abstract
Boehmeria tricuspis (Hance)Makino is a perennial herbaceous plant which contains ex-ceptionally high amounts of polyphenols,polysaccharides,and other secondary metabolites that interfere with DNA isolation.Six methods were compared for the extraction of genomic DNA from young leaves of Boehmeria tricuspis,and we expected to find a correct method which can make us quickly obtained high quality genomic DNA.The total DNAs extracted by different methods were detected by agarose gel elec-trophoresis,the ultraviolet absorbency,the amplification of DNAs with SSR primer and restriction endo-nuclease reaction.The results indicate the solution of genomic DNA extracted by method 1 (Tian -Gen kit)contains impurities and little DNA,method 2(simple CTAB)produces a lot of impurities,method 3 (silica gel -CTAB)and method 4(phenol extracting -CTAB)can’t extract genomic DNAs,and lots of proteins or salts in the solution of genomic DNA is obtained by method 5 (high -salt predeimpurity -CTAB),while the method 6(glucose predeimpurity -CTAB)is the best way to obtain high quality ge-nomic DNA and can meet the requirement for PCR,restriction endonuclease reaction and other DNA mo-lecular experiments.This study found a generally simple,low cost and safe way to remove impurities such as polysaccharides in plant DNA extracted process,which laid the foundation of follow -up study of ram-ie in other molecular biology.关键词
悬铃叶苎麻/多糖/改良 CTAB 法/DNA 提取Key words
Boehmeria tricuspis (Hance)Makino/polysaccharides/modified CTAB/DNA extraction分类
农业科技引用本文复制引用
董志雪,唐蜻,程超华,信鹏飞,高春生,李育君,臧巩固,赵立宁..悬铃叶苎麻基因组 DNA 的六种提取方法比较[J].中国麻业科学,2015,(5):233-238,6.基金项目
国家麻类产业体系分子育种（CARS －19－E04）等资助 （）
