中国农业科技导报Issue(2)：83-88,6.DOI:10.3969/j.issn.1008-0864.2013.02.13
外壳蛋白基因片段介导的高抗TuMV研究
Research of High Resistance to Turnip Mosaic Virus Mediated by Coat Protein Gene Segment
摘要
Abstract
In order to get stable high resistance to turnip mosaic virus (TuMV), a 377-nucleotide (nt) segment near 3′conserved region of TuMV coat protein (CP) gene was chosen to construct hairpin RNAi vector, and transformed into Arabidopsis thaliana. The transgenic plants were inoculated with TuMV isolate BJ-C4, which was of high pathogenicity and mainly epidemic in Beijing area. Three of the 8 transgenic lines showed hyper-resistance to TuMV. Quantitative PCR results revealed that the accumulation of virus was hardly to detect in highly resistant transgenic plants.关键词
芜菁花叶病毒/外壳蛋白基因/发卡结构/RNA干扰/抗性Key words
turnip mosaic virus/coat protein gene/hairpin structure/RNAi/resistance分类
生物科学引用本文复制引用
曾钢,叶艳英,曹鸣庆,马荣才,唐乐尘,姚磊..外壳蛋白基因片段介导的高抗TuMV研究[J].中国农业科技导报,2013,(2):83-88,6.基金项目
国家863计划项目(2008AA10Z154) （2008AA10Z154）
北京市自然科学基金(6092010)资助 （6092010）
