中国农业科技导报Issue(2)：193-199,7.DOI:10.3969/j.issn.1008-0864.2013.02.30
基于PCR的非酶连型基因组步移技术研究进展
Progress on Ligation-independent PCR-mediated Approaches for Genomic Walking
摘要
Abstract
PCR-mediated approaches for genomic walking (or chromosome walking) are routinely used for cloning flanking sequences of the known DNA regions, which is an important technology for genomic research. According to the principles, they can be categorized into two types of ligation-dependence and ligation-independence. At present, numerous approaches of the later, each with distinct features, have been rapidly developed. Nevertheless, regarding the binding patterns of walking primers on DNA templates, they can be overall grouped into the degenerate primer-based or specific sequence-dependent subsets. Following this viewpoint, this paper summarized the major or representative techniques and the recent developments of ligation-independent, PCR-mediated genomic walking, so as to provide a methodological reference for the relevant researches.关键词
非酶连/PCR/基因组步移/染色体步移Key words
ligation-independence/PCR/genomic walking/chromosome walking分类
生物科学引用本文复制引用
李旭娟,陈杨玲,王海波,龚明,邹竹荣..基于PCR的非酶连型基因组步移技术研究进展[J].中国农业科技导报,2013,(2):193-199,7.基金项目
国家自然科学基金项目(31060160 （）
31160169) （）
云南省教育厅科研基金项目(2010Z087)资助 （2010Z087）
