中国农业科技导报Issue(3)：84-90,7.DOI:10.3969/j.issn.1008-0864.2013.03.12
陆地棉 LTP 家族基因的克隆与表达分析
Cloning and Expression Pattern Analysis of Gossypium hirsutum Lipid Transfer Protein Gene Family
摘要
Abstract
Long-chain fatty acids could activate cotton fiber initiation and elongation by regulating intracellular ethylene signal transduction in cotton ovule. In order to fully understand how fatty acids are transferred to the surface of fiber cells and how many genes are involved in this process, we cloned 11 LTP genes after genome-wide search and analyzed their expression patterns during fiber’s development by quantitative RT-PCR. The results showed that there are differences between expressions of LTP6, LTP7, LTP9 and LTP11, and these 4 genes might be closely related to cotton fiber development at the original stage. We also cloned Gh-LTP6 promoter by Genome Walking technique and analyzed its motif, results showed there are mutiple MYB binding site. These results might lay a solid foundation for intensive studying the role of LTP genes in the process of cotton fiber development.关键词
陆地棉/LTP家族/基因克隆/棉纤维发育/脂质转运蛋白/启动子Key words
Gossypium hirsutum/LTP family/gene cloning/cotton fiber development/lipid transfer protein/promoter分类
农业科技引用本文复制引用
韩慧超,黄逸群,王劲,左开井..陆地棉 LTP 家族基因的克隆与表达分析[J].中国农业科技导报,2013,(3):84-90,7.基金项目
国家新品种培育转基因重大专项(2011ZX08005-001) （2011ZX08005-001）
国家自然基金项目(31071458)资助。 （31071458）
