摘要
Abstract
Objective To investigate the correlation between rs 2282679 A/C polymorphism in vitamin D -binding protein (VDBP) gene and chronic obstructive pulmonary disease (COPD) .Methods Enrolled 120 COPD patients who were admitted into Harrison International Peace Hospital in Hengshui from March 2012 to May 2014 as COPD group.Also enrolled 112 health males who received physical examination in the same period as control group.ELISA was employed to detect serum 25 ( OH) D, and TaqMan-PCR was used to detect the allele frequency and genotype distribution of rs 2282679 A/C in VDBP gene.Results The 25 ( OH) D levels of control group and COPD group were ( 55.1 ±5.8 ) nmol/L and ( 37.3 ±2.3 ) nmol/L respectively, with control group higher than COPD group (t=31.220, P<0.001) .Control group and COPD group were significantly different in allele frequency and genotype distribution of rs 2282679 A/C in VDBP gene ( P<0.05 ) .Control group and COPD group were significantly different in 25 (OH) D levels of AA, AC and CC genotypes (P<0.05) .In control group and COPD group, subjects of AA, AC and CC genotypes were significantly different in 25 (OH) D level (P<0.05);subjects of AC and CC genotype were lower than subjects of AA genotype , and subjects of CC genotype were lower than subjects of AC genotype ( P<0.05) .After the adjustment of age and smoking indexes , AA genotype is 18.3%lower than AC genotype in susceptibility to COPD 〔OR =0.374, 95%CI (0.197, 0.708), P =0.002〕; AA genotype is 35.8% lower than CC genotype in susceptibility to COPD 〔OR=0.260, 95%CI (0.106, 0.640), P=0.002〕; AC genotype is 16.7%lower than CC genotype in susceptibility to COPD 〔OR=0.201, 95%CI (0.849, 4.744), P=0.109〕 .Conclusion rs2282679 A/C polymorphism in VDBP gene is significantly associated with COPD.CC genotype is related to the liability of COPD and is closely related with 25 ( OH) D, and it may be a mutation site for the screening of COPD liability.关键词
肺疾病, 慢性阻塞性/维生素D结合蛋白质/基因/多态性, 单核苷酸Key words
Pulmonary disease , chronic obstructive/Vitamin D -binding protein/Genes/Polymorphism, single nucleotide分类
医药卫生
