中国全科医学Issue(32)：3948-3951,4.DOI:10.3969/j.issn.1007-9572.2015.32.013
何首乌二苯乙烯苷对拟痴呆大鼠学习记忆和海马组织 CA1区脑啡肽酶及低密度脂蛋白相关受体1表达的影响
Influence of TSG on the Learning and Memory and the Expression of Neprilysin and Low Density Lipoprotein Receptor 1 in Hippocampus CA1 Area of Dementia Mimetic Rats
摘要
Abstract
Objective To investigage the influence of TSG on the learning and memory and the expression of neprilysin (NEP)and low density lipoprotein receptor 1(LRP - 1)in hippocampus CA1 area of dementia mimetic rats. Methods Using random number table method,60 rats were randomly divided into Group A to Group F,with 10 rats in each group. Group A was the control group which was administrated with 0. 9% and 300 mg/ kg sodium chloride solution by one time per day for 6 weeks consecutively. Group B - F were administrated with back subcutaneous injection of 300 mg/ kg D - galactose by one time per day for 6 weeks consecutively,in order to build AD models. While building models,Group C underwent gavage by 0. 9 mg/ kg donepezil hydrochloride,and Group D - F underwent gavage by TSG of 0. 05,0. 10 and 0. 20 g/ kg respectively by one time per day for 12 weeks consecutively. The space memory ability of the rats were observed by Morris water maze test,during which place navigation test was undertaken in the first 5 days to train the learning ability of rats,and spatial probe test was undertaken on day 6 to record the angel of entering water and the number of times of crossing platform. The memory of passive avoidance was observed by step - through tests,in which the rats were trained on day 1,and tests were conducted 24 hours later with the number of times of entering dark room and latency period recoded within 5 minutes. Blood was sampled from the eye sockets of the rats after anesthesia,and the serum level of Aβ1 - 42 was detected using radioimmunoassay. Brains of the rats were obtained after killing the rats,and after immumohistochemical staining,the expression of NEP and LRP - 1 of hippocampus CA1 area were measured by accumulate optical density. Results The groups were significantly different( P ﹤ 0. 05)in the angle of entering water and the number of times of crossing platform. Group B had bigger angle of entering water than Group A and Group C - F(P ﹤ 0. 05);Group B had lower number of times of crossing platform than Group A and Group C - F(P ﹤ 0. 05). The groups were significantly different(P ﹤ 0. 05)in latency period and the number of times of receiving electric shock. Group B had shorter latency period than Group A and Group C - F(P ﹤ 0. 05);Group B has higher number of times of electric shock than Group A and Group E(P ﹤ 0. 05). The groups were significantly different( P ﹤ 0. 05)in the serum Aβ1 - 42 level and the accumulated optical density of NEP and LRP - 1 in hippocampus CA1 area. Group B was higher than Group A and Group E - F in serum Aβ1 - 42 level(P ﹤ 0. 05);Group B was lower than Group A and Group D - F in the accumulated optical density(P ﹤0. 05);Group B was lower than Group A and Group E in the accumulated optical density of LRP - 1(P ﹤ 0. 05). Conclusion TSG can significantly improve the learning and memory of dementia mimetic ratsm,improve the expression of NEP and LRP -1 in hippocampus CA1 area and promote the degradation and transferring of Aβ.关键词
阿尔茨海默病/二苯乙烯苷/淀粉样β肽类/脑啡肽酶/低密度脂蛋白受体相关蛋白-1Key words
Alzheimer disease/TSG/Amyloid beta - peptides/Neprilysin/Low density lipoprotein receptor -related protein - 1分类
医药卫生引用本文复制引用
李小黎,刘晓梅,赵瑞珍,田青..何首乌二苯乙烯苷对拟痴呆大鼠学习记忆和海马组织 CA1区脑啡肽酶及低密度脂蛋白相关受体1表达的影响[J].中国全科医学,2015,(32):3948-3951,4.基金项目
教育部高等学校博士学科专项科研基金资助项目 （）
