中国全科医学Issue(35)：4317-4319,4329,4.DOI:10.3969/j.issn.1007-9572.2015.35.011
自体淋巴细胞宫腔灌注对反复种植失败患者妊娠结局的影响
Effect of Intrauterine Infusion of Lymphocytes on the Outcome of Pregnancy in Patients With Recurrent Implantation
摘要
Abstract
Objective To investigate the effect of intrauterine infusion of lymphocytes on the outcome of pregnancy of frozen embryo transfer(FET)in patients with recurrent implantation failure(RIF). Methods We enrolled 74 RIF patients who received FET in the reproductive center in Changzhou Women and Children Health Hospital Affiliated to Nanjing Medical University from January 2014 to January 2015. Patients who required to have intrauterine infusion of lymphocytes three days before the next FET cycle were assigned into treatment group ( n =38 ), and patients who didn't have intrauterine infusion of lymphocytes were assigned into control group(n=36). After autologous lymphocytes were prepared using lymphocyte separation medium,and intrauterine infusion treatment was conducted. Intima preparation plan,number of transferred embryo during FET cycle and pregnancy outcomes of the two groups were observed. Results The two groups were not significantly different in age, years of infertility,FSH,BMI,number of high -quality transferred embryos and type of infertility( P ﹥0. 05 for all). In treatment group, there were 33 ( 86. 8%) cases of artificial cycle and 5 ( 13. 2%) cases of natural cycle;the average thickness of intima was ( 9. 5 ± 1. 2 ) mm, the average number of transferred embryo was ( 2. 11 ± 0. 45 ), and the average number of high quality transferred embryo was(1. 51 ± 0. 50). In control group,there were 30(83. 3%)cases of artificial cycle and 6(16. 7%)cases of natural cycle;the average thickness of intima was(9. 0 ± 1. 7)mm,the average number of transferred embryo was(2. 17 ± 0. 44),and the average number of high quality transferred embryo was(1. 64 ± 0. 76). The two groups were not significantly different in intima preparation plan(χ2 =0. 18,P=0. 67),the average thickness of intima(t=1. 35,P=0. 18),the average number of transferred embryo(t = -0. 58,P =0. 55),and the average number of high quality transferred embryo(t= -0. 83,P=0. 41). For treatment group,the embryo implantation rate of the treatment group was 22. 5%(18/80),and the clinical pregnancy rate was 34. 2%(13/38);for control group,the embryo implantation rate for control group was 7. 0%(5/71),and the clinical pregnancy rate was 8. 3%(3/36). Treatment group was higher than control group in the embryo implantation rate and clinical pregnancy rate(χ2 =7. 388,P=0. 007;χ2 =7. 791,P=0. 010). Conclusion Autologous lymphocyte infusion in intrauterine in FET cycle may be an effective approach to improve the embryo implantation rate and clinical pregnancy rate in patients with RIF.关键词
体外受精/胚胎移植/反复种植失败/淋巴细胞/宫腔灌注Key words
Fertilization in vitro/Embryo transfer/Recurrent implantation failure/Lymphocytes/Intrauterine administration分类
医药卫生引用本文复制引用
曹芳,黄晓阳,于春梅,杨海燕,王宇峰,周伟,吴娟芬,陈莉..自体淋巴细胞宫腔灌注对反复种植失败患者妊娠结局的影响[J].中国全科医学,2015,(35):4317-4319,4329,4.基金项目
南京医科大学科技发展基金重点项目 （）
