中国人兽共患病学报Issue(10)：903-907,918,6.DOI:10.3969/j.issn.1002-2694.2015.10.002
抗弓形虫水通道蛋白多肽抗体的制备及鉴定
Preparation and identification of anti-TgAQP peptide antibody
摘要
Abstract
In this study ,we intended to prepare anti‐Toxoplasma gondii aquaporin (TgAQP) peptide antibody which was used to the application in the detection of the aquaporin expression and its subcellular localization of Toxop lasma gondii (T .gondii) ME49 strain .The B cell peptide antigen was designed based on the TgAQP amino acids sequence .After the pep‐tide antigen was conjugated to the KLH ,the fusion antigen was injected into New Zealand rabbits to prepare polyclonal anti‐body ,followed by identification of ELISA ,Western‐blotting and immunofluorescence assays .The ELISA showed that the titer of anti‐TgAQP antibody was about 1∶40 000 .Western blotting revealed the specific affinity of the antigen to polyclonal anti‐body at 29 .9 kDa protein T .gondii .The protein detected by the indirect immunofluorescence assays was distributed in the cy‐toplasm of the parasite .Thus far ,the anti‐TgAQP polyclonal antibody was successfully prepared ,providing a useful tool for further study of biological function and metabolic characteristics of TgAQP .关键词
弓形虫/水通道蛋白/多肽抗体/亚细胞定位Key words
Toxop lasma gondii/aquaporin/peptide antibody/subcellular localization分类
医药卫生引用本文复制引用
张佳凤,郝文波,肖斌,廖小青,罗树红..抗弓形虫水通道蛋白多肽抗体的制备及鉴定[J].中国人兽共患病学报,2015,(10):903-907,918,6.基金项目
国家自然科学基金（No ．81171608）Supported by the National Natural Science Foundation of China （）
