中国人兽共患病学报Issue(10)：919-922,4.DOI:10.3969/j.issn.1002-2694.2015.10.005
梅毒螺旋体 TprF 蛋白 B 细胞表位的预测与鉴定
Prediction and identification of B-cell epitopes of Treponema pallidum repeat protein F
摘要
Abstract
To predict and identify the dominant B‐cell epitopes of conserved region of Treponema pallidum repeat protein F (TprFN ) and provide the basis for development of polyvalent epitope‐based syphilis vaccine ,the amino acid sequence of TprFN was obtained from GenBank and analyzed with comprehensive meta‐analysis Mobyle ,ABCpred and IEDB online software .The peptides containing predicted epitopes were artificially synthesized . To obtain and measure the titers of antibodies against TprFN ,New Zealand rabbits were immunized with recombinant protein TprFN expressed in E .coli and identified by Western blot (WB) .Sera from TprFN‐immunized rabbits ,syphilis patients ,and normal human and normal rabbits were used to deter‐mine the immunoreactivity and specificity of 7 predicted peptides of TpFN by indirect ELISA .Comprehensive meta‐analysis of online software showed that P1 (43‐62AA) ,P2(57‐71AA) ,P3(81‐88AA) ,P4(89‐103AA) ,P5(125‐138AA) ,P6(231‐251AA) and P7(268‐279AA) might be the B‐cell epitopes .A protein was expressed in a soluble form and identified as TpFN by WB .The ELISA indicated that P1 and P3 were active with TprFN‐immunized rabbit sera and syphilis patient sera but not with negative control sera .These results indicate that P1 and P3 are the potential dominant B‐cell epitopes .关键词
梅毒螺旋体/T pF/B细胞表位Key words
Treponema pallidum/TprF/B-cell epitope分类
医药卫生引用本文复制引用
曹龙古,凌晖,蔡恒玲,赵飞骏,欧阳丹明,陈苏芳,吴移谋,曾铁兵..梅毒螺旋体 TprF 蛋白 B 细胞表位的预测与鉴定[J].中国人兽共患病学报,2015,(10):919-922,4.基金项目
国家自然科学基金（No ．81273322，81373230）；湖南省教育厅资助科研项目（No ．13C876）；郴州市科技局资助科研项目（No ．2012CJ126）；特殊病原体防控湖南省重点实验室资助项目（湘科计字［2014］5号）@@@@Supported by the National Natural Science Foundation of China （Nos ．81273322 and 81373230），the Scientific Research Fund of Hunan Provincial Education Department （No ．13C876），the Chenzhou Municipal Science and Technology Bureau （No ．2012CJ126），and the Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control Foundation （）
