中国人兽共患病学报Issue(10)：952-956,962,6.DOI:10.3969/j.issn.1002-2694.2015.10.012
猪带绦虫重组 BCG-TSOL18疫苗构建及其表达
Construction and expression of a recombinant BCG-TSOL18 vaccine of Taenia solium
摘要
Abstract
We constructed a recombinant Bacillus Calmette‐Guerin(BCG)‐TSOL18 vaccine of Taenia solium and observed the expression of the TSOL18 gene in BCG .The TSOL18 gene of Taenia solium was obtained from the recombinant plasmid pGEX‐TSOL18 by digestion method and cloned into Escherichia coli (E .coli)‐mycobacterium shuttle plasmid pMV261 to con‐struct the recombinant plasmid pMV261‐TSOL18 of Taenia solium ,and the recombinant plasmid was identified by restriction enzyme digestion ,PCR and DNA sequencing .Then ,the recombinant plasmid was transformed into BCG by electroporation to construct the recombinant BCG‐TSOL18 vaccine of Taenia solium ,and the vaccine was identified by PCR .The expression of the TSOL18 gene in BCG was identified by SDS‐PAGE and Western blot .The 393 bp TSOL18 gene fragment was successfully obtained by restriction enzyme digestion .Restriction enzyme digestion ,PCR and DNA sequencing suggested that the recombi‐nant plasmid pMV261‐TSOL18 of Taenia solium was successfully constructed .PCR confirmed that the recombinant plasmid pMV261‐TSOL18 of Taenia solium was successfully transformed into BCG ,suggesting that the recombinant BCG‐TSOL18 vaccine of Taenia solium was successfully constructed .SDS‐PAGE showed that the relative molecular mass (Mr) of TSOL18 target protein was approximately 14 .7 kD .Results of western blot showed the TSOL18 target protein could be recognized by rabbit antiserum or cysticercosis swine serum .The recombinant BCG‐TSOL18 vaccine of Taenia solium was successfully con‐structed .The TSOL18 gene of Taeniasolium was successfully expressed in BCG and the expressed TSOL18 recombinant pro‐tein had specific antigenicity .This result would lay a foundation for further study of the vaccine .关键词
猪带绦虫/重组BCG-TSOL18疫苗/构建/表达Key words
Taenia solium/recombinant BCG-TSOL18 vaccine/construction/expression分类
医药卫生引用本文复制引用
杨凤娇,周必英..猪带绦虫重组 BCG-TSOL18疫苗构建及其表达[J].中国人兽共患病学报,2015,(10):952-956,962,6.基金项目
贵州省科技厅社会发展攻关项目（黔科合SY字［2011］3038号）Supported by the Social Research Project of Science and Technology Department of Guizhou Province （）
