中国人兽共患病学报Issue(10)：957-962,6.DOI:10.3969/j.issn.1002-2694.2015.10.013
铜绿假单胞菌耐药基因分析及多位点序列遗传分型
Antibiotic-resistant genes and multilocus sequencing typing of Pseudomonas aeruginosa
摘要
Abstract
We investigated the antibiotic‐resistant genes and genetic diversity of Pseudomonas aeruginosa from patients in hospital ,the smear samples from hospital and clinic environment ,and from medical staff’ hands respectively in 2011‐2012 in Nanshan District of Shenzhen .Polymerase chain reaction were used to detect the 20 kinds of antibiotic‐resistant genes (TEM , VEB,CARB,OXA,SHV,PER,GES,GTX,SPM,GIM,IMP,VIM,DHA,oprD,Aac(6′)‐Ⅰ ,Aac(6′)‐Ⅱ ,Aac (3′)‐Ⅰ ,A ac(2″)‐Ⅰ ,qacE1‐sull and int‐Ⅰ) .Multilocus sequencing typing was used to analyze the clonal complexes .The 11 kinds resistant genes TEM ,SHV ,IMP ,DHA ,Aac(6′)‐Ⅰ ,Aac(6′)‐Ⅱ ,Aac(3′)‐Ⅰ ,Aac(2″)‐Ⅰ ,qacE1‐sull ,int‐Ⅰand oprD were detected ,for the positive rates respectively ,and which were 8 .1% ,6 .4% ,4 .8% ,9 .7% ,4 .8% ,14 .5% ,9 .7% , 56 .5% ,8 .1% ,and 8 .1% ;the loss rate of oprD gene was 61 .2% .The 19 antibiotic resistance gene profiles existed in 52 Pseudomonas aeruginosa strains .Multilocus sequencing typing found 39 sequence types and 5 clonal complexes in 62 Pseudo‐monas aeruginosa strains ,CC244 and ST856 were dominant .There were some differences of antibiotic resistance gene profiles between different samples ,the Pseudomonas aeruginosa strains from patients carried multiple resistant genes .In our research , the Pseudomonas aeruginosa had the genetic diversity and the dominant clonal complexes existed .关键词
铜绿假单胞菌/多重耐药/耐药基因/聚合酶链反应/多位点序列分子分型/克隆群Key words
Pseudomonas aeruginosa/multi-drug resistance/antibiotic-resistant genes/polymeras chain reaction/multilo-cus sequencing typing/clonal complexes分类
医药卫生引用本文复制引用
袁梦,袁月明,陈宏彬,罗锦雁,俞慕华,段永翔..铜绿假单胞菌耐药基因分析及多位点序列遗传分型[J].中国人兽共患病学报,2015,(10):957-962,6.基金项目
深圳市科技计划项目（201103296）Supported by Shenzhen Science and Technology Plan Project （）
