中国蔬菜Issue(11)：51-54,4.
SMART技术构建的辣椒疫霉菌与辣椒互作AD-cDNA文库
Construction of AD-cDNA Library for Interaction ofPhytophthora capsici and Pepper in SMART Technology
摘要
Abstract
In order to identify thePhytophthora capsici stress induced genes of pepper, a double-hybrid cDNA library ofPhytophthora capsici infected pepper leaves was constructed using SMART technology. The results showed that the cDNA library contained 3.6×106 independent clones, and the recombination rate was 88%. Insert fragments ranged from 300 bp to 2 000 bp, and the average length of the library was about 800 bp. These data demonstrate that the library is qualified, and able to provide important genetic resources for molecular breeding.关键词
辣椒疫霉菌/SMART技术/双杂交cDNA文库Key words
Phytophthora capsici/SMART technology/Double-hybrid cDNA library引用本文复制引用
周志国,明月梅..SMART技术构建的辣椒疫霉菌与辣椒互作AD-cDNA文库[J].中国蔬菜,2015,(11):51-54,4.基金项目
河北省科技计划项目（12226306），河北省高校食药用菌应用技术研发中心项目 （）
