中国水产科学Issue(1)：108-115,8.DOI:10.3724/SP.J.1118.2013.00108
饲料脂肪水平对梭鱼脂肪沉积、脂肪代谢酶及抗氧化酶活性的影响
Effects of dietary lipid levels on fat deposition, lipid metabolize en-zyme and antioxidantic activities of Chelon haematocheilus
摘要
Abstract
We evaluated the effects of dietary lipid levels on fat deposition, lipid metabolism enzymes, and antioxidant activity in Chelon haematocheilus. The fish (mean weight 5.40±0.16 g) were assigned to one of six treatment groups (triplicate, n=20 fish/replicate) and fed one of six isonitrogenous diets containing 2.71%, 5.79%, 8.23%, 11.85%, 14.39%, or 16.91%lipid for 60 d. Increases in the dietary lipid level were associated with a sig-nificant increase in the hepatosomatic and viscerosomatic indexes (P<0.05). Condition factor was highest in the group fed 8.23%lipid, and was significantly higher than the group fed 2.71%lipid (P<0.05). There was a signifi-cant increase in fat deposition in the whole body and liver as the level of dietary lipid increased. The whole body lipid content was highest in the group fed 14.39% lipid whereas liver lipid was highest in the group fed 16.91%lipid. Dietary lipid had a significant effect on lipid metabolic enzymes (P<0.05). Lipoprotein lipase, HL, and the general esterase all increased with an increase in dietary lipid levels. The levels were significantly higher in the group fed 16.91% lipid than in the groups fed ≤8.23% lipid (P<0.05). Conversely, the levels of malic dehydro-genase tended to decrease with increasing dietary lipid levels. Levels were significantly higher in the group fed 2.71%lipid than in all other groups (P<0.05). Blood fat also increased as the level of dietary lipid increased, but there was no change in TG, CHO, and EFA. High density lipoproteins were significantly higher in the group fed 16.91% lipid than in those fed 2.71% lipid (P<0.05). Liver superoxide dismutase (SOD) activity increased then decreased as the level of dietary lipid increased. The point of inflection was around 8.23%lipid. Malondialdehyde (MDA) content was significantly higher in the fish fed 16.91% lipid than that of other five groups(P<0.05). In summary, our results suggest that higher dietary lipid increases the hepatosomatic and viscerosomatic indexes as well as fat deposition. The liver is the primary place for fat deposition, so was more sensitive than muscle. The higher levels of dietary lipid also increased lipid enzymatic activity and blood fat content. Optimal dietary lipid levels enhanced antioxidant activity in C. haematocheilus, although excess lipid levels were associated with a de-crease in antioxidant activity.关键词
梭鱼/脂肪水平/脂肪沉积/脂肪代谢/抗氧化Key words
Chelon haematocheilus/lipid level/fat deposition/lipid metabolism/digestive enzyme/antioxidantion分类
农业科技引用本文复制引用
张春暖,王爱民,刘文斌,杨文平,於叶兵,吕林兰,黄金田,齐志涛..饲料脂肪水平对梭鱼脂肪沉积、脂肪代谢酶及抗氧化酶活性的影响[J].中国水产科学,2013,(1):108-115,8.基金项目
江苏省2011年度科技型企业技术创新资金项目(BC2011423) （BC2011423）
