中国水产科学Issue(2)：250-256,7.DOI:10.3724/SP.J.1118.2013.00250
胭脂鱼受精过程的细胞学观察
Cytological study on fertilization process of Myxocyprinus asiaticus
摘要
Abstract
The insemination process of Myxocyprinus asiaticus egg was studied using artificial fertilization, his-tology, ultrastructural technology methods etc. The observation showed that there is only one micropyle in shell of egg’s animal pole of M. asiaticus. Micropylar apparatus of M. asiaticus belongs to the type of deep concave and short channel. At water temperature of 17-18℃, sperm passed through micropylar apparatus at 3 s after insemina-tion and entered ovum at 5 s. Sperm star light was formed at 10 s after insemination. After 15 min after insemina-tion ovum was in late time of second meiotic division and second polar body appeared. Ovum was in telophase after 20 min and second meiotic division was formed. Male and female pronucleus was fused after 90 min. Ovum was in metaphase of first karyokinesis at 140 min after insemination. At 180 min after insemination first karyoki-nesis was over and second cell was formed. The results showed that in micropylar apparatus of mature egg there is no micropylar cell and other stuff, which is similar to that of most other teleost. The inner diameter of micorpylar apparatus is 2.42 µm and near to the diameter of sperm head. There are many spines in inner walls of anterior mi-cropylar apparatus and holes are arranged in spiral way, which was one of the reasons for monospermy. This study aims to provide basic data for the research of artificial reproduction technology of M. asiaticus.关键词
胭脂鱼/精孔器/受精过程/细胞学Key words
Myxocyprinus asiaticus/micropylar apparatus/fertilization/cytology分类
农业科技引用本文复制引用
李萍,刘晓蕾,李勇,江星,金丽,张耀光..胭脂鱼受精过程的细胞学观察[J].中国水产科学,2013,(2):250-256,7.基金项目
重庆市自然科学基金重点项目(CSTS,2009BA1053) （CSTS,2009BA1053）
