中国水产科学Issue(2)：299-307,9.DOI:10.3724/SP.J.1118.2013.00299
渗透胁迫对中华绒螯蟹胚胎生化组成及消化酶活性的影响
Developmental changes of biochemical composition and digestive enzyme activity in the eggs of the Chinese mitten crab, Eriocheir sinensis, under osmotic stress
摘要
Abstract
Embryonic development of the Chinese mitten crab, Eriocheir sinensis, is completed in estuaries where they are exposed to frequent and abrupt changes in environmental salinity. Biochemical composition (carbohy-drates, proteins and lipids) content and digestive enzyme (amylase, lipase, pepsin and trypsin) activity were de-termined in three developmental stages (gastrula, eyespot and pre-hatching stage) of E. sinensis eggs under hyper-or hypo-osmotic stress. Increases in pepsin and trypsin activity (P<0.05) and decreases in protein (P<0.05), lipid content (P>0.05) and lipase activity (P<0.05) in the eggs of the control group were noticed during embryonic de-velopment. Total carbohydrate content and amylase activity increased at the eyespot stage (P<0.05), whereas they reduced during the pre-hatching stages (P<0.05). The protein content during all three developmental stages de-creased under hyper-osmotic stress while it increased under hypo-osmotic stress (P<0.05). Total lipid content was unaffected by hyper-osmotic stress at any developmental stage, whereas it reduced significantly at the pre-hatching stage under hypo-osmotic stress (P<0.05). Total carbohydrate content did not show any significant variation after osmotic stress. Hyper-osmotic stress reduced enzyme activity at the three developmental stages; however, hypo-osmotic stress enhanced it, with the exception of reducing amylase activity in the eyespot stage (P<0.05) and trypsin activity in the pre-hatching stage under both hyper- and hypo-osmotic stress (P>0.05). With the exception of lipase, the effect of salinity changes on digestive enzymes was only statistically significant during the gastrula stage (P<0.05). We suggest that proteins and lipids are important structural materials of embryonic development and provide energy for this process. Osmotic stress changed the activity of digestive enzymes, which further af-fects the use of yolk substances, and consequently affects embryonic development. Extremely high or low salinity in estuaries potentially affects the embryonic development of E. sinensis, especially under salt-water encroach-ment.关键词
中华绒螯蟹/胚胎发育/盐度/渗透胁迫/消化酶/生化成分Key words
Eriocheir sinensis/embryonic development/salinity/osmotic stress/digestive enzymes/biochemical composition分类
农业科技引用本文复制引用
王瑞芳,庄平,黄晓荣,冯广朋,章龙珍,陆建学,王海华,王妤..渗透胁迫对中华绒螯蟹胚胎生化组成及消化酶活性的影响[J].中国水产科学,2013,(2):299-307,9.基金项目
国家973计划项目(2010CB429005) （2010CB429005）
国家公益性行业(农业)科研专项(200903048-07,201203065) （农业）
中央级公益性科研院所基本科研业务费专项(东海水产研究所2011M08) （东海水产研究所2011M08）
