中国水产科学Issue(2)：402-410,9.DOI:10.3724/SP.J.1118.2013.00402
饲料中赖氨酸水平对克氏原螯虾生长、体组成与消化酶活性的影响
Effect of dietary lysime levels on grow, body composition and diges-tive enzyme activity of red swamp crayfish (Procambarus clarkii)
摘要
Abstract
To determine the optimal dietary lysine requirement of red swamp crayfish, Procambarus clarkii, we evaluated the effects of dietary lysine levels on growth performance, feed utilization, body composition, muscle amino acid composition, and digestive enzyme activity of this species. Seven isonitrogenous (28.26% crude pro-tein) and isocaloric (17.15 kJ·g−1 gross energy) diets were formulated with protein sources from the mixture of casein, gelatin, wheat gluten, and crystalline amino acid (AA) mixture. The measured lysine levels of the experi-mental diets were 0.76%, 1.00%, 1.22%, 1.47%, 1.69%, 1.89% and 2.18%. Each diet was fed to four groups of 12 crayfish with an initial body weight of (7.60±0.01) g in floating net cages (60 cm×60 cm×60 cm). The crayfish were fed three times daily for 60 d. The results indicated that weight gain rate (WGR), specific growth rate (SGR), and feed efficiency ratios (FER) all increased significantly with increasing dietary lysine levels (P<0.05), and their maximum values were attained at 1.69%, 1.69%, and 1.22%, respectively. However, they all decreased with fur-ther increasing dietary lysine levels. Survival rate ranged from 89.58% to 97.92%, and showed no significant dif-ference among all the dietary treatments (P>0.05). Whole-body moisture content of crayfish fed 1.22% dietary lysine was significantly lower than that of crayfish fed 0.76% dietary lysine (P<0.05), but showed little difference from that of the other treatments (P>0.05). The highest whole-body protein and lipid content was observed in crayfish fed 1.89% and 1.69 dietary lysine, respectively. They were both significantly higher than that of crayfish fed 0.76% lysine (P<0.05), but showed little difference with that of the other treatments (P>0.05). No significant difference was found in whole-body ash content, muscle composition, muscle AA contents, or digestive enzyme activity among all the treatments (P>0.05). The second-order regression analysis of WGR against dietary lysine levels indicated that the optimal dietary lysine requirement of red swamp crayfish (Procambarus clarkii) was 1.66% of diet (5.87% of dietary protein).关键词
克氏原螯虾/赖氨酸需求量/生长性能/体组成/消化酶活性Key words
Procambarus clarkii/lysine requirement/growth performance/body composition/digestive enzyme activity分类
农业科技引用本文复制引用
张微微,徐维娜,王莹,刘文斌,鲁康乐,李向飞,杨维维..饲料中赖氨酸水平对克氏原螯虾生长、体组成与消化酶活性的影响[J].中国水产科学,2013,(2):402-410,9.基金项目
江苏省水产三项工程(PJ2010-56) （PJ2010-56）
农业部公益性行业科研专项(201003070) （201003070）
