中国水产科学Issue(2)：411-418,8.DOI:10.3724/SP.J.1118.2013.00411
基于 P-糖蛋白基因表达评价尼罗罗非鱼体内恩诺沙星代谢“首过效应”
Association between permeability glycoprotein expression and enro-floxacin metabolism to evaluate the first-pass effect in Oreochomis niloticus Linn
摘要
Abstract
A fluorescence quantitative PCR method for detecting the P-glycoprotein gene in Oreochomis niloticus Linn was established to evaluate the first-pass effects. After the oral gavage at a dosage of 40 mg·kg-1 for O. niloticus, the relative expression quantity of P-glycoprotein in the intestinal and liver tissues was associated with the concentration of enrofloxacin in the plasma. The results showed that P-glycoprotein fragments (127 bp) were successfully amplified in both the intestinal and liver tissues of O. niloticus by using specific primers. The concentrations of enrofloxacin were determined by HPLC. Enrofloxacin can be rapidly absorbed in the intestine and infiltrated into the plasma. The concentration versus time in the intestine, liver and plasma of O. niloticus were well described by a two-department open model with first-order absorption. The relative expression quantity of P-glycoprotein of both intestinal and liver tissues in the first hour were higher than the control group (P<0.05) while that of the intestine in the second hour was significantly higher (P<0.01). The relative expression quantity of P-glycoprotein of liver tissues in the second hour were higher than the control group (P<0.05). These results sug-gest that P-glycoprotein may be involved in the enrofloxacin metabolism process in O. niloticus, providing a novel model for the potential utility of gene expression and drug metabolism studies in aquatic animals.关键词
P-糖蛋白/尼罗罗非鱼/恩诺沙星/首过效应/药物代谢Key words
P-glycoprotein/Oreochomis niloticus Linn/enrofloxacin/first-pass effect/drug metabolism分类
农业科技引用本文复制引用
胡鲲,程钢,吕利群,章海鑫,王会聪,阮记明,陈力,房文红,杨先乐..基于 P-糖蛋白基因表达评价尼罗罗非鱼体内恩诺沙星代谢“首过效应”[J].中国水产科学,2013,(2):411-418,8.基金项目
国家863计划项目(2011AA10A216) （2011AA10A216）
公益性农业行业专项项目(201203085) （201203085）
现代农业产业技术体系(CARS-46-12)和农业部海洋与河口渔业资源及生态重点开放实验室开放基金课题(开-09-06) （CARS-46-12）
国家科技基础条件平台项目 （）
上海海洋大学博士科研启动基金资助项目(A-2400-12-0000341) （A-2400-12-0000341）
