中国水产科学Issue(3)：481-489,9.DOI:10.3724/SP.J.1118.2013.00481
长牡蛎糖原磷酸化酶基因SNPs与生长性状和糖原含量的相关性分析
Single nucleotide polymorphisms in glycogen phosphorylase gene and their association with growth performance and glycogen content in Pacific oyster Crassostrea gigas
摘要
Abstract
Glycogen phosphorylase plays a key role in the metabolism of glycogen, a molecule critical for the growth and development of oysters. We evaluated the effect of polymorphisms in the Crassostrea gigas glycogen phosphorylase gene (Cg-GPH) on growth and glycogen content. We tested the association between single nucleo-tide polymorphisms (SNPs) in coding regions of the gene and growth performance (shell height, shell length, shell depth, total weight and soft-tissue weight) and glycogen content using 322 oysters from five full-sib families. We identified 82 SNPs in the 1 940 bp Cg-GPH gene, including 63 SNPs in the exon region, one SNP locus located in the 5′-UTR, and 18 SNPs in the 3′-UTR. The average density of SNPs in the coding region was one per 25 bp. Five SNPs were significantly associated with growth performance (P<0.05), but there was no association between the SNPs and glycogen content. Furthermore, among the six SNP haplotypes constructed using the five SNPs above, the total weight of the individuals with haplotype H6 (CTGAT) of Cg-GPH was significantly higher than those with the other five haplotypes(P<0.05), suggesting this haplotype may be the most advantageous in terms of weight gain in C.gigas. Our results provide basic information for future genetic improvement of growth perform-ance in C.gigas.关键词
长牡蛎/糖原磷酸化酶基因/生长性状/糖原/SNPsKey words
Crassostrea gigas/glycogen phosphorylase gene/growth performance/glycogen/SNPs分类
农业科技引用本文复制引用
刘思玮,李琪,于红,孔令锋..长牡蛎糖原磷酸化酶基因SNPs与生长性状和糖原含量的相关性分析[J].中国水产科学,2013,(3):481-489,9.基金项目
国家973计划项目(2010CB126406) （2010CB126406）
国家863计划项目(2012AA10A405-6) （2012AA10A405-6）
国家海洋公益性行业科研专项(200905020) （200905020）
