中国水产科学Issue(4):713-721,9.DOI:10.3724/SRJ.1118.2013.00713
4个虹鳟养殖品系的分子鉴定和遗传多样性分析
Identification and genetic diversity analysis of four rainbow trout strains using microsatellites
摘要
Abstract
We evaluated the genetic diversity among four rainbow trout strains at 30 microsatellite loci. In addition, we compared the genetic diversity in these strains with that of samples collected in 2005 to evaluate the genetic differentiation attributable to selection pressure. All 30 microsatellite loci are suitable markers of genetic diversity in the four rainbow trout strains. The mean number of alleles in the four strains varied from 3.77 to 5.80; the mean number of effective alleles varied from 2.746 1 to 3.630 3; the mean expected heterozygosity ranged from 0.590 6 to 0.722 1,the mean observed heterozygosity ranged from 0.587 5 to 0.786 1,and the mean polymorphism information content ranged from 0.535 0 to 0.647 0. All four rainbow trout strains exhibited a high level in genetic diversity. Analysis of the genetic distance revealed that Finland rainbow trout and Norway rainbow trout cluster together and the two Golden trout cluster together. When compared with the samples acquired in 2005,Norway rainbow trout and American golden trout have significantly lower allele numbers, effective allele numbers, and polymorphism information content(P0.01), but there was no difference for Finland rainbow trout.关键词
虹鳟/微卫星/种质鉴定/遗传多样性/遗传参数变动Key words
Oncorhynchus mykiss/microsatellite/germplasm identification/genetic diversity/genetic parameter variation分类
农业科技引用本文复制引用
..4个虹鳟养殖品系的分子鉴定和遗传多样性分析[J].中国水产科学,2013,(4):713-721,9.基金项目
国家科技基础条件平台项目(2006DKA30470-005) (2006DKA30470-005)