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4个虹鳟养殖品系的分子鉴定和遗传多样性分析

中国水产科学Issue(4):713-721,9.
中国水产科学Issue(4):713-721,9.DOI:10.3724/SRJ.1118.2013.00713

4个虹鳟养殖品系的分子鉴定和遗传多样性分析

Identification and genetic diversity analysis of four rainbow trout strains using microsatellites

摘要

Abstract

We evaluated the genetic diversity among four rainbow trout strains at 30 microsatellite loci. In addition, we compared the genetic diversity in these strains with that of samples collected in 2005 to evaluate the genetic differentiation attributable to selection pressure. All 30 microsatellite loci are suitable markers of genetic diversity in the four rainbow trout strains. The mean number of alleles in the four strains varied from 3.77 to 5.80; the mean number of effective alleles varied from 2.746 1 to 3.630 3; the mean expected heterozygosity ranged from 0.590 6 to 0.722 1,the mean observed heterozygosity ranged from 0.587 5 to 0.786 1,and the mean polymorphism infor­mation content ranged from 0.535 0 to 0.647 0. All four rainbow trout strains exhibited a high level in genetic di­versity. Analysis of the genetic distance revealed that Finland rainbow trout and Norway rainbow trout cluster to­gether and the two Golden trout cluster together. When compared with the samples acquired in 2005,Norway rainbow trout and American golden trout have significantly lower allele numbers, effective allele numbers, and polymorphism information content(P0.01), but there was no difference for Finland rainbow trout.

关键词

虹鳟/微卫星/种质鉴定/遗传多样性/遗传参数变动

Key words

Oncorhynchus mykiss/microsatellite/germplasm identification/genetic diversity/genetic parameter variation

分类

农业科技

引用本文复制引用

..4个虹鳟养殖品系的分子鉴定和遗传多样性分析[J].中国水产科学,2013,(4):713-721,9.

基金项目

国家科技基础条件平台项目(2006DKA30470-005) (2006DKA30470-005)

中国水产科学

OA北大核心CSCDCSTPCD

1005-8737

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