中国水产科学Issue(4)：824-831,8.DOI:10.3724/SP.J.1118.2013.00824
桑沟湾不同养殖区水体微生物群落结构特征
Effect of mariculture activities on bacterial community structure in the water of Sanggou Bay
摘要
Abstract
We evaluated the bacterial community structure in water collected from several mariculture zones in Sanggou Bay in summer 2007 using PCR-DGGE. Some of the bands were shared by two or more stations, while others were unique to a particular location. Based on the DGGE profiles, we distinguished four areas, including a no-culture area (outer bay), kelp farming area (bay-mouth), integrated mariculture area (majority of the area in the middle of the bay), and a mollusk monoculture or fish farming area (bottom of the bay). The highest similarity in the bacterial community structure was between the scallop and oyster culture areas (94%), while the lowest was between the no-culture and mollusk monoculture or fish farming area (41%). Sequence analysis of twelve major bands from the DGGE profiles revealed that these sequences were highly similar to those of Actinobacteria, Fir-micutes, and Proteobacteria, including class Alpha and Gamma proteobacteria. The similarity of the nucleotide sequences ranged from 92% to 98%. Roseobacter, which can remove nitrogen-containing pollutants, was only present in the fish farming area. Our results suggest that seaweed culture had a lower impact on the environment then shellfish monoculture and fish culture. Thus practicing IMTA can reduce the degree of pollution from shell-fish and fish monoculture.关键词
微生物群落结构/DGGE/桑沟湾/养殖区Key words
bacterial community structure/DGGE/Sanggou Bay/mariculture分类
农业科技引用本文复制引用
..桑沟湾不同养殖区水体微生物群落结构特征[J].中国水产科学,2013,(4):824-831,8.基金项目
国家自然科学基金资助项目(40776090 （）
40976073) （）
国家科技支撑计划项目(2011BAD13B02) （2011BAD13B02）
国家海洋局海洋公益性行业科研专项(201205031,201305043) （201205031,201305043）
