中国水稻科学Issue(6)：587-594,8.DOI:10.3969/j.issn.1001-7216.2015.06.004
水稻窄叶突变体 nal10的鉴定与基因精细定位
Identification and Fine Mapping of a Narrow Leaf Mutant nal10 in Rice
摘要
Abstract
A novel rice mutant narrow leaf 10 (nal10 )was identified from an EMS (ethyl methane sulphonate) mutagenized population of Wuyunjing 7.The mutant was characterized by narrow leaves and smaller architecture phenotype at the seedling stage,and dwarf,narrow leaves,deformed flowers and male sterility at the reproduction stage.Cyto-anatomical analysis indicated that the mutant had fewer large and small veins compared with the wild-type, which may account for the narrow leaf phenotype.The genetic analysis indicated the narrow leaf trait was controlled by a recessive nuclear gene.Using F2 population derived from the cross of nal10 and Taizhongbendi 1 ,three closely linked SSR markers RM1287,RM5 62 and RM5 638 on chromosome 1 were obtained by the bulked segregant analysis method. With the developed new markers,the gene was finally fine-mapped to an interval of 55 .2 kb between the STS markers PK83 and PK78,where a total of eight ORFs were found.RT-PCR analysis indicated the expression levels of auxin biosynthesis,signal transduction and transport related genes were all down-regulated,suggesting NAL10 is a new narrow leaf gene involving in the auxin metabolism.These results laid a foundation for further cloning of the gene and enrich the genetic regulation network of leaf development in rice.关键词
水稻/窄叶/NAL10/基因定位Key words
rice/narrow leaf/NAL10/gene mapping分类
生物科学引用本文复制引用
方云霞,祝阳舟,董国军,曾大力,张光恒,郭龙彪,胡江,钱前,朱丽,潘江杰,余海平,薛大伟,饶玉春,王小虎,石珍源,张栋..水稻窄叶突变体 nal10的鉴定与基因精细定位[J].中国水稻科学,2015,(6):587-594,8.基金项目
国家自然科学基金资助项目(31201194,91335105) （31201194,91335105）
