中国实验动物学报2015，Vol.23Issue(5)：495-499,5.DOI:10.3969/j.issn.1005-4847.2015.05.010
碱性鞘磷脂酶基因剔除鼠的基因型和表型特征
Genotypic and phenotypic characteristics of alkaline sphingomyelinase knockout mice
摘要
Abstract
Objective To determine the genotypic and phenotypic characteristics of alkaline sphingomyelinase (alk-SMase) knockout (KO) mice and to provide the foundation data of this animal model for further study of the role of alk-SMase in colon cancer .Methods DNA was extracted from the tail tissue of wild type , heterozygous , homozygous KO mice, respectively.PCR analysis was used for genotyping .HE staining, confocal microscopy, and mass spectrometry were used for the detection of morphology , alk-SMase expression and sphingolipid metabolites in liver and intestine of the mice . Results Compared with the wild type and heterozygous mice , the homozygous KO mice showed that no changes occurred in the appearance and body weight , there was only one band (247bp) appeared on the genotyping , the thickness in small intestinal mucosa was significantly increased with a lower expression level of alk -SMase, and the amount of sphingolipid me-tabolites in the intestine and liver was changed , i.e.increase of SM and S1P, and reduction of ceramide .Conclusions Our findings demonstrate that the homozygous KO mice have specific genotype and phenotype that do not affect their growth.These mice will provide an ideal animal model for further study of alk-SMase functions.关键词
碱性鞘磷脂酶/基因剔除小鼠/基因型/表型Key words
alk-SMase/Knockout mouse/Genotype/Phenotype分类
生物科学引用本文复制引用
杨俐萍,朱顺星,闵娅兰,刘焕良,钱红燕,陈橼,朱菁烨,邵晶晶,雒润华..碱性鞘磷脂酶基因剔除鼠的基因型和表型特征[J].中国实验动物学报,2015,23(5):495-499,5.基金项目
江苏省创新创业人才基金(项目号:苏人才2013-41). （项目号:苏人才2013-41）
