中国实验动物学报2015，Vol.23Issue(5)：513-516,4.DOI:10.3969/j.issn.1005-4847.2015.05.014
脂多糖联合 MPTP 诱导的小鼠慢性帕金森病模型的评价
Evaluation of a mouse model of Parkinson' s disease induced by lipopolysaccharide plus MPTP
摘要
Abstract
Objective The aim of this study was to establish a mouse model of chronic Parkinson ' s disease in-duced by systemic administration of lipopolysaccharide plus 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP), and to study the changes of behavioral manifestation , numbers of dopaminergic neurons in the substantia nigra pars compacta . Methods Twenty C57BL mice were randomly divided into 2 groups:the saline control group and model group .The mice in the model group received three intraperitoneal (i.p.) injections of LPS (0.25 mg/kg), once daily for three consecutive days.Four hours following the final LPS injection , the mice received one subcutaneous injection of low-dose MPTP (25 mg/kg).The mice of control group were injected with the same volume of saline .Eight weeks later, the motor ability of the mice was evaluated by footprint test and rotarod test .The tyrosine hydroxylase ( TH)-positive cells were observed by immu-nohistochemical analysis .Results Compared with the control group , the scores of behavioral test were significantly lower , numbers of TH immunoreactive cells were significantly less in the Parkinson ' s model group ( P<0.05 ) .Conclusions Behavioral manifestation ,number of dopaminergic neurons in the substantia nigra are significantly changed in the mouse models of Parkinson ' s disease produced by repeated injection of LPS plus MPTP , suggesting that this chronic animal model can be used in the experimental study for pathogenesis and therapy of Parkinson ' s disease .关键词
LPS/MPTP/帕金森病/小鼠模型/酪氨酸羟化酶Key words
Lipopolysaccharide/LPS/MPTP/Parkinson's disease(PD)/Rat model/Tyrosine hydroxylase分类
生物科学引用本文复制引用
史肖锦,屈洪党..脂多糖联合 MPTP 诱导的小鼠慢性帕金森病模型的评价[J].中国实验动物学报,2015,23(5):513-516,4.基金项目
蚌埠医学院研究生创新计划项目(Byycx1406) （Byycx1406）
教育厅重点项目(KJ2014A163). （KJ2014A163）
