中国比较医学杂志Issue(10):7-10,4.DOI:10.3969.j.issn.1671.7856.2015.010.002
斑马鱼 uba6和 uba7突变体的构建与鉴定
Construction and identification of zebrafish uba6 and uba7 mutants by TALEN
摘要
Abstract
Objective To construct zebrafish uba6 and uba7 mutants.Method In-dels were introduced into coding regions of zebrafish uba6 and uba7 gene by TALEN technology, and mutation types were identified by sequencing.Results We obtained two uba6 and three uba7 mutants with different small fragment deletions resulting in reading frame shifts .Conclusion These uba6 and uba7 mutants provided starting materials for the study of zebrafish uba6 and uba7 gene functions.关键词
斑马鱼/uba6基因/uba7基因/TALENKey words
Zebrafish/uba6/uba7/TALEN分类
医药卫生引用本文复制引用
丁慧美,阮华,黄红辉..斑马鱼 uba6和 uba7突变体的构建与鉴定[J].中国比较医学杂志,2015,(10):7-10,4.基金项目
国家自然科学基金面上项目(31071286);西南大学中央高校基本科研业务费(XDJK2014A013;2362014xk02)。 ()