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斑马鱼 uba6和 uba7突变体的构建与鉴定

丁慧美 阮华 黄红辉

中国比较医学杂志Issue(10):7-10,4.
中国比较医学杂志Issue(10):7-10,4.DOI:10.3969.j.issn.1671.7856.2015.010.002

斑马鱼 uba6和 uba7突变体的构建与鉴定

Construction and identification of zebrafish uba6 and uba7 mutants by TALEN

丁慧美 1阮华 1黄红辉1

作者信息

  • 1. 淡水鱼类资源与生殖发育教育部重点实验室,三峡库区生态环境与生物资源省部共建国家重点实验室培育基地,西南大学生命科学学院,重庆 400715
  • 折叠

摘要

Abstract

Objective To construct zebrafish uba6 and uba7 mutants.Method In-dels were introduced into coding regions of zebrafish uba6 and uba7 gene by TALEN technology, and mutation types were identified by sequencing.Results We obtained two uba6 and three uba7 mutants with different small fragment deletions resulting in reading frame shifts .Conclusion These uba6 and uba7 mutants provided starting materials for the study of zebrafish uba6 and uba7 gene functions.

关键词

斑马鱼/uba6基因/uba7基因/TALEN

Key words

Zebrafish/uba6/uba7/TALEN

分类

医药卫生

引用本文复制引用

丁慧美,阮华,黄红辉..斑马鱼 uba6和 uba7突变体的构建与鉴定[J].中国比较医学杂志,2015,(10):7-10,4.

基金项目

国家自然科学基金面上项目(31071286);西南大学中央高校基本科研业务费(XDJK2014A013;2362014xk02)。 ()

中国比较医学杂志

OA北大核心CSTPCD

1671-7856

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