中国比较医学杂志Issue(10)：11-16,6.DOI:10.3969.j.issn.1671.7856.2015.010.003
降钙素基因相关肽转基因小鼠的建立及血压动态分析
Establishment and dynamic analysis of blood pressure in calcitonin gene-related peptide transgenic mice
摘要
Abstract
Objective To establish calcitonin gene-related peptide α( CGRPα) andβ( CGRPβ) transgenic mice and analyze dynamic changes of blood pressure using this transgenic mouse model in hypertension research .Methods The transgenic vector was constructed by inserting the human CGRPαand CGRPβgene into the down steam of chicken β-actin promoter , respectively .The transgenic mice were created by microinjection .The genotype of transgenic line was identified by PCR and the expression level of target gene was determined by Western blot .Dynamic changes of blood pressure was measured by noninvasive blood pressure meter .Results One line of human CGRPαC57BL/6J transgenic mice with high levels of CGRPαexpression in the heart , lung, kidney and liver tissues was established .One line of human CGRPβC57BL/6J transgenic mice with high levels of CGRPβexpression in the lung and liver tissues was established . Mice overexpressing the human CGRPαwere normotensive , whereas mice overexpressing the human CGRPβwere hypotensive at 12 months of age .Conclusions CGRPαand CGRPβtransgenic mice may serve as an animal model for the mechanism research on the roles of CGRP on peripheral vascular resistance and hypertension resistance .关键词
CGRP/转基因/外周血管阻力/高血压抵抗Key words
CGRP/Transgene/Peripheral vascular resistance/Hypertension resistance分类
医药卫生引用本文复制引用
鲍丹,董伟,张旭,刘宁,高珊,张海涛,高翔,葛文平,吕丹..降钙素基因相关肽转基因小鼠的建立及血压动态分析[J].中国比较医学杂志,2015,(10):11-16,6.基金项目
国家自然科学基金（31301932）。 （）
