中国动物传染病学报Issue(3)：1-7,7.
免疫后发病仔猪中伪狂犬病毒的分离和鉴定
IDENTIFICATION AND CHARACTERIZATION OF A PSEUDORABIES VIRUS ISOLATED FROM A DEAD PIGLET BORN TO VACCINATED SOW
摘要
Abstract
The newborn pigs in many herds have suffered from a disease that is clinically similar to pseudorabies in China since 2011. In late 2012, the disease outbreak occurred in a herd in Jiangsu province that received PRV vaccination. Brain tissue of a dead piglet was collected and examined for PRV by PCR. Two pairs of primers specific for PRV gB and gE were designed and used to amplify the corresponding DNA segments. The resulting DNA segments were sequenced and shown to share very high homology with known PRV. A pseudorabies virus was isolated from this dead piglet. The homogenated brain tissue was inoculated to Vero cells. Classical cytopathogenic effects of herpesvirus appeared in Vero cells at 24 hours post-inoculation. The virus was cloned by six rounds of plaque purification in Vero cells. The infected cells showed positive reaction with PRV antibodies in IFA. Therefore, the isolated virus was confirmed to be PRV. The PRV isolate grew well in Vero cells with the titer up to 107.43 TCID50/mL. The mice infected with this isolate developed unbearable itching and died later. The LD50 of the isolate was lower than that of PRV S strain. Genetic analysis of gE gene revealed that the isolate was different from classical virulent strain and vaccine strain.关键词
伪狂犬病毒/分离/鉴定Key words
Pseudorabies virus/isolation/identification分类
农业科技引用本文复制引用
童武,张青占,郑浩,刘飞,姜一峰,单同领,周艳君,童光志..免疫后发病仔猪中伪狂犬病毒的分离和鉴定[J].中国动物传染病学报,2013,(3):1-7,7.基金项目
国家生猪现代产业技术体系项目 （）
