中国动物传染病学报Issue(3)：8-12,5.
马立克氏病病毒 RB1B 株 UL14蛋白原核表达及多抗血清制备
PROKARYOTIC EXPRESSION OF UL14 GENE OF MDV STRAIN RB1B AND PREPARATION OF ANTISERUM TO UL14 PROTEIN
摘要
Abstract
To investigate the biological function of UL14 protein during the course of MDV infection, UL14 gene from CEF cells infected with RB1B strain was amplified by PCR. UL14 gene was cloned into pET32a (+) vector and expressed in E.coli BL21 (DE3). Recombinant His-UL14 protein expressed in E.coli was soluble. The BALB/c mice were immunized with recombinant UL14 protein and resulting antiserum was used in IFA. The specific inflorescence was visualized in CEF cells infected with MDV strain RB1B .关键词
马立克氏病病毒/UL14 蛋白/原核表达Key words
Marek's disease virus/UL14 protein/prokaryotic expression分类
农业科技引用本文复制引用
徐文财,刘秋,秦爱建,胡序明,吴庚华,孙苹,钱琨,邵红霞,金文杰..马立克氏病病毒 RB1B 株 UL14蛋白原核表达及多抗血清制备[J].中国动物传染病学报,2013,(3):8-12,5.基金项目
国家自然科学基金项目（31272560）；教育部创新团队项目（IRT0978）；江苏省优势学科和江苏省教育厅重大基础研究项目 （）
