中国动物传染病学报Issue(4)：12-17,6.
番鸭源鸭肝炎病毒CH12株的分离与全基因组序列分析
ISOLATION AND SEQUENCE ANALYSIS OF DUCK HEPATITIS VIRUS STRAIN CH12 FROM DOMESTIC MUSCOVY DUCKLING
黄显明 1张小飞 1毛火云 1尹秀凤 1丁美娟 1王伟 1靳宇田1
作者信息
- 1. 南京天邦生物科技有限公司，南京211102
- 折叠
摘要
Abstract
Duck hepatitis virus (DHV) strain CH12 was isolated from a diseased domestic Muscovy duckling in Zhejiang Province and characterized in disease reproduction in host animals, ELD50 determination and duck embryo neutralization. The strain CH12 was identified as DHV-1 (genotype A). The complete genome sequence of the strain CH12 was amplified in RT-PCR. The full length of its genome was 7690 nt, including 626 nt of 5' untranslated region (UTR), an open reading frame of 6747 nt and 317 nt of 3' untranslated region. The pairwise percent identity of the genome sequences between strain CH12 and other genotype A strains were higher than that between strain CH12 and genotype B or genotype C strains. Phylogenetic and evolutionary analysis on complete genome, polyprotein, VP0, VP3, VP1, 2C and 3D indicated that strain CH12 had a very close relationship with DHV genotype A.关键词
鸭肝炎病毒/CH12/病毒分离/全基因组Key words
Duck hepatitis virus (DHV)/CH12/virus isolation/complete genome分类
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黄显明,张小飞,毛火云,尹秀凤,丁美娟,王伟,靳宇田..番鸭源鸭肝炎病毒CH12株的分离与全基因组序列分析[J].中国动物传染病学报,2013,(4):12-17,6.
