中国动物传染病学报Issue(4)：37-43,7.
基于微小隐孢子虫重组CP15/60蛋白的间接ELISA检测方法的建立及上海市猪隐孢子虫感染情况调查
DEVELOPMENT OF INDIRECT ELISA WITH RECOMBINANT CP15/60 OFCRYPTOSPORIDIUM PARVUM AND SEROLOGICAL SURVEY OF PIG CRYPTOSPORIDIOSIS IN SHANGHAI
摘要
Abstract
To develop sensitive and specific serological detection method of pigCryptosporidium infection and understand the situation ofCryptosporidium infection in pigs in Shanghai, an indirect ELISA method were developed to detectCryptosporidium infection in pigs with the recombinant CP15/60 (rCP15/60) protein as diagnostic antigen. The reproducibility, sensitivity and specificity of the method were evaluated. The situation of pigsCryptosporidium infection were serological detected with the developed method. The result showed that comparing with Nested PCR detection results, the negative and positive coincidence, sensitivity and specificity were 83.3%, 100% and 80%, respectively. The coefficient of variability of reproducibility was≤12.5%. Total of 194 (56.89%) sera were positive in 341 sera collected from pigs in field in Shanghai. These results indicated that the method was suitable to be used as laboratory primary diagnosis and field serological survey of porcine cryptosporidiosis.关键词
微小隐孢子虫/重组CP15/60/间接ELISA/调查Key words
Cryptosporidiumparvum/recombinant CP15/60/indirect ELISA/survey分类
农业科技引用本文复制引用
刘宇轩,米荣升,黄燕,于慧珠,胡盼,詹婷婷,周金林,陈兆国..基于微小隐孢子虫重组CP15/60蛋白的间接ELISA检测方法的建立及上海市猪隐孢子虫感染情况调查[J].中国动物传染病学报,2015,(4):37-43,7.基金项目
国家科技重大专项项目（2012ZX10004220）；2015年国家畜禽产品质量安全风险评估项目（GJFP201500803)；家畜疫病家畜疫病病原生物学国家重点实验室开放基金课题（SKLVEB2013KFKT017）；上海市科技兴农重点攻关项目 （）
