中国听力语言康复科学杂志Issue(1)：19-23,5.DOI:10.3969/j.issn.1672-4933.2013.01.003
单次放射线照射早期Balb/c小鼠畸变产物耳声发射变化和耳蜗外毛细胞Prestin蛋白表达的实验观察
Experimental Observation of the Influence of Single-dose Radiation on the Distortion Product Otoacoustic Emission and the Prestin Expressions in the Cochlear Outer Hair Cells of Balb/c Mice in the Early Period after Radiation Exposure
摘要
Abstract
Objective To study the effect of radiation exposure on the distortion product otoacoustic emission(DPOAE) and Prestin expression in the cochlear outer hair cells of Balb/c mice in the early period after single-dose radiation exposure and to examine the possible mechanism of radiation causing sensorineural hearing loss(SNHL). Methods The Balb/c mouse models with outer hair cells exposed to radiation were established. The DPOAE amplitudes of the mice were detected and compared before, 3 days and 7 days after exposure to a single dose of 16Gy radiation. The Western Blot and reverse transcription-polymerase chain reaction(RT-PCR) assays were used to analyze the changes of expression of Prestin and its mRNA in the cochleae of mice. Results The Balb/c mouse models with SNHL after radiation exposure were successfully established. The DPOAE amplitudes of the mice on the 3rd and 7th day after radiation exposure were lower than those before radiation exposure at 3,4,6,8,10 and 12 kHz and the amplitudes on the 7th day were lower than those on the 3rd day at most frequencies. The expressions of Prestin and its mRNA after radiation exposure were parallel down-regulated as compared with those before exposure and the expressions of Prestin and its mRNA on the 7th day after radiation exposure were lower than those on the 3rd day. Conclusion The auditory function of the mice was affected in the early period after radiation exposure. The mechanism of SNHL after radiation exposure may be correlated with Prestin expression abnormalities in the outer hair cells of cochleae in the early period after radiation exposure.关键词
Balb/c小鼠/放射治疗/外毛细胞/Prestin蛋白/畸变产物耳声发射/感音神经性聋Key words
Balb/c mice/Radiotherapy/Outer hair cell/Prestin protein/Distortion product otoacoustic emission(DPOAE)/Sensorineural hearing loss引用本文复制引用
赵向东,梁勇,任陈,袁亚维..单次放射线照射早期Balb/c小鼠畸变产物耳声发射变化和耳蜗外毛细胞Prestin蛋白表达的实验观察[J].中国听力语言康复科学杂志,2013,(1):19-23,5.基金项目
广东省科技计划对外合作项目(项目编号:2010B050700021) （项目编号:2010B050700021）
